Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/43963
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Type: Journal article
Title: Biochemical characterisation of the esterase activities of wine lactic acid bacteria
Author: Matthews, A.
Grbin, P.
Jiranek, V.
Citation: Applied Microbiology and Biotechnology, 2007; 77(2):329-337
Publisher: Springer-Verlag
Issue Date: 2007
ISSN: 0175-7598
1432-0614
Statement of
Responsibility: 
Angela Matthews, Paul R. Grbin and Vladimir Jiranek
Abstract: Esters are an important group of volatile compounds that can contribute to wine flavour. Wine lactic acid bacteria (LAB) have been shown to produce esterases capable of hydrolysing ester substrates. This study aims to characterise the esterase activities of nine LAB strains under important wine conditions, namely, acidic conditions, low temperature (to 10°C) and in the presence of ethanol (2–18% v/v). Esterase substrate specificity was also examined using seven different ester substrates. The bacteria were generally found to have a broad pH activity range, with the majority of strains showing maximum activity close to pH 6.0. Exceptions included an Oenococcus oeni strain that retained most activity even down to a pH of 4.0. Most strains exhibited highest activity across the range 30–40°C. Increasing ethanol concentration stimulated activity in some of the strains. In particular, O. oeni showed an increase in activity up to a maximum ethanol concentration of around 16%. Generally, strains were found to have greater activity towards short-chained esters (C2–C8) compared to long-chained esters (C10–C18). Even though the optimal physicochemical conditions for enzyme activity differed from those found in wine, these findings are of potential importance to oenology because significant activities remained under wine-like conditions.
Keywords: Esters; Oenococcus; Lactobacillus; Pediococcus; Hydrolysis
Description: The original publication can be found at www.springerlink.com
RMID: 0020073423
DOI: 10.1007/s00253-007-1173-8
Appears in Collections:Agriculture, Food and Wine publications

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