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Type: Journal article
Title: The protein tyrosine phosphatase Pez regulates TGFβ, epithelial–mesenchymal transition, and organ development
Other Titles: The protein tyrosine phosphatase Pez regulates TGFbeta, epithelial-mesenchymal transition, and organ development
Author: Belle, L.
Wadham, C.
Crocker, L.
Lardelli, M.
Khew-Goodall, Y.
Citation: Journal of Cell Biology, 2007; 178(7):1223-1235
Publisher: Rockefeller Univ Press
Issue Date: 2007
ISSN: 0021-9525
Organisation: Centre for the Molecular Genetics of Development
Statement of
Leila Wyatt, Carol Wadham, Lesley A. Crocker, Michael Lardelli, and Yeesim Khew-Goodall
Abstract: Epithelial–mesenchymal transition (EMT), crucial during embryogenesis for new tissue and organ formation, is also considered to be a prerequisite to cancer metastasis. We report here that the protein tyrosine phosphatase Pez is expressed transiently in discrete locations in developing brain, heart, pharyngeal arches, and somites in zebrafish embryos. We also find that Pez knock-down results in defects in these organs, indicating a crucial role in organogenesis. Overexpression of Pez in epithelial MDCK cells causes EMT, with a drastic change in cell morphology and function that is accompanied by changes in gene expression typical of EMT. Transfection of Pez induced TGFβ signaling, critical in developmental EMT with a likely role also in oncogenic EMT. In zebrafish, TGFβ3 is co- expressed with Pez in a number of tissues and its expression was lost from these tissues when Pez expression was knocked down. Together, our data suggest Pez plays a crucial role in organogenesis by inducing TGFβ and EMT.
Keywords: Cell Nucleus; Epithelial Cells; Embryo, Nonmammalian; Mesoderm; Animals; Zebrafish; Dogs; Humans; Transforming Growth Factor beta; Zebrafish Proteins; Transcription Factors; Oligonucleotides, Antisense; RNA, Messenger; Signal Transduction; Gene Expression Regulation, Developmental; Protein Transport; Embryonic Development; Organogenesis; Phenotype; Smad4 Protein; Transforming Growth Factor beta1; Transforming Growth Factor beta2; Transforming Growth Factor beta3; Protein Tyrosine Phosphatases; Protein Tyrosine Phosphatases, Non-Receptor
Description: Published online September 24, 2007
Rights: © 2007 Rockefeller University Press
RMID: 0020073085
DOI: 10.1083/jcb.200705035
Appears in Collections:Molecular and Biomedical Science publications
Centre for the Molecular Genetics of Development publications

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