Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Ghrelin selectively reduces mechanosensitivity of upper gastrointestinal vagal afferents|
|Citation:||American Journal of Physiology-Gastrointestinal and Liver Physiology, 2007; 292(5):G1376-G1384|
|Publisher:||Amer Physiological Soc|
|Amanda J. Page, James A. Slattery, Catherine Milte, Rhianna Laker, Tracey A O'Donnell, Camilla Dorian, Stuart M Brierley, and L. Ashley Blackshaw|
|Abstract:||Ghrelin is a peptide released from gastric endocrine cells that has an orexigenic effect via a vagal pathway. Here we determine the effect of ghrelin on mechanosensitivity of upper-intestinal vagal afferent fibers in ferret and mouse. The responses of gastroesophageal vagal afferents to graded mechanical stimulation were determined in vitro before and during application of ghrelin to their peripheral endings. Three types of vagal afferent were tested: tension receptors responding to circumferential tension, mucosal receptors responding only to mucosal stroking, and tension/mucosal (TM) receptors in ferret esophagus that responded to both stimuli. In the mouse, ghrelin did not significantly affect the response of mucosal receptors to mucosal stroking with calibrated von Frey hairs. However, it significantly reduced responses of tension receptors to circumferential tension (P < 0.005; two-way ANOVA) by up to 40%. This inhibition was reversed by the ghrelin receptor antagonist [d-Lys-3]-growth hormone-releasing peptide (GHRP)-6. In the ferret, ghrelin significantly reduced the response of mucosal and TM receptors to mucosal stroking with calibrated von Frey hairs. Surprisingly, ghrelin did not significantly alter the response to circumferential tension in either tension or TM receptors. RT-PCR analysis indicated that both ghrelin and its receptor are expressed in vagal afferent cell bodies in mouse nodose ganglia. In conclusion, ghrelin selectively inhibits subpopulations of mechanically sensitive gastroesophageal vagal afferents; there is also potential for ghrelin release from vagal afferents. However, the subpopulation of afferents inhibited differs between species. These data have broad implications for ghrelin's role in food intake regulation and reflex control of gastrointestinal function.|
|Keywords:||Esophagus; Stomach; Nodose Ganglion; Afferent Pathways; Vagus Nerve; Mechanoreceptors; Animals; Ferrets; Mice; Peptide Hormones; Oligopeptides; Receptors, G-Protein-Coupled; Female; Ghrelin; Receptors, Ghrelin|
|Description:||Copyright © 2007 by the American Physiological Society|
|Appears in Collections:||Medicine publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.