Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/44126
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Type: Journal article
Title: Effect of cytokine and antigen stimulation on peripheral blood lymphocyte syndecan-1 expression
Author: Manakil, J.
Seymour, G.
Bartold, P.
Citation: Molecular Oral Microbiology, 2007; 22(4):272-276
Publisher: Blackwell Munksgaard
Issue Date: 2007
ISSN: 0902-0055
1399-302X
Statement of
Responsibility: 
J. F. Manakil, G. J. Seymour, P. M. Bartold
Abstract: <h4>Introduction</h4>Cytokines are not only produced by activated lymphocytes but also interact with a number of cell-surface molecules on the same cells. Syndecan-1 is one such cell-surface molecule, which has the capacity to bind a variety of growth factors as well as cytokines. The aim of this study was to examine the effects of transforming growth factor beta (TGF-beta), interleukin-1 (IL-1), IL-2, IL-4, lipopolysaccharide (LPS) from Porphyromonas gingivalis and tetanus toxoid on syndecan-1 expression by B and T lymphocytes.<h4>Methods</h4>B and T lymphocytes were obtained from the peripheral blood of healthy donors. Following exposure to the above growth factors, cytokines and antigens, syndecan-1 expression was determined by flow cytometry.<h4>Results</h4>Subjects could be categorized as high or low expressors of syndecan-1. In the high-responder group TGF-beta1 alone resulted in a significant increase in syndecan-1 expression by both B and T cells. None of the other cytokines and antigens produced a significant response. When analysed in combination, TGF-beta1 in combination with IL-2, IL-4, P. gingivalis LPS and tetanus toxoid all produced significant increases in syndecan-1 expression by B cells. For T cells, combinations of TGF-beta1 with IL-2 and tetanus toxoid resulted in increased syndecan-1 expression.<h4>Conclusions</h4>Both B and T lymphocytes synthesize the cell-surface proteoglycan syndecan-1 and its expression can be modulated by TGF-beta1, either alone or in combination with IL-2, IL-4 and LPS from P. gingivalis and tetanus toxoid. While these may reflect general responses under inflammatory conditions their biological significance requires further investigation.
Keywords: Lymphocytes
Cells, Cultured
Humans
Recombinant Proteins
Antigens, Bacterial
Cytokines
Drug Combinations
Analysis of Variance
Regression Analysis
Lymphocyte Activation
Gene Expression Regulation
Adult
Middle Aged
Female
Male
Transforming Growth Factor beta1
Syndecan-1
DOI: 10.1111/j.1399-302X.2007.00356.x
Appears in Collections:Aurora harvest 6
Dentistry publications

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