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Type: Journal article
Title: Regulation of the CYP27B1 5 '-flanking region by transforming growth factor-beta in ROS 17/2.8 osteoblast-like cells
Author: Turner, A.
Dwivedi, P.
May, B.
Morris, H.
Citation: The Journal of Steroid Biochemistry and Molecular Biology, 2007; 103(3-5 Sp Iss):322-325
Part of: 13th Workshop on Vitamin D (Victoria, British Columbia, Canada, April 2006) / edited by R. Bouillon, A.W. Norman and J.R. Pasqualini
Publisher: Pergamon-Elsevier Science Ltd
Issue Date: 2007
ISSN: 0960-0760
Statement of
Andrew G. Turner, Prem P. Dwivedi, Brian K. May and Howard A. Morris
Abstract: The biologically active form of vitamin D, 1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)), regulates osteoblast proliferation and differentiation. Production of 1,25(OH)(2)D(3) is catalysed by the enzyme 25-hydroxyvitamin D(3)-1alpha-hydroxylase (CYP27B1). Though highly expressed in the kidney, the CYP27B1 gene is also expressed in non-renal tissues including bone. It is hypothesised that local production of 1,25(OH)(2)D(3) by osteoblasts plays an autocrine or paracrine role. The aim of this study was to investigate what factors regulate expression of the CYP27B1 gene in osteoblast cells. ROS 17/2.8 osteoblast cells were transiently transfected with plasmid constructs containing the 5'-flanking sequence of the human CYP27B1 gene fused to a luciferase reporter gene. Cells were treated with either parathyroid hormone (PTH), 1,25(OH)(2)D(3), transforming growth factor-beta (TGF-beta) or insulin-like growth factor-1 (IGF-1) and luciferase activity was measured 24h later. The results showed that 1,25(OH)(2)D(3) did not alter expression of the reporter construct, however treatment with PTH, IGF-1 and TGF-beta decreased expression by 18, 53 and 58% respectively. The repressive action of TGF-beta was isolated to the region between -531 and -305bp. These data suggest that expression of the 5'-flanking region for the CYP27B1 gene in osteoblast cells may be regulated differently to that previously described in kidney cells.
Keywords: Cell Line, Tumor
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Transforming Growth Factor beta
Gene Expression Regulation, Enzymologic
5' Flanking Region
Description: Crown copyright © 2006 Published by Elsevier Ltd.
DOI: 10.1016/j.jsbmb.2006.12.041
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Molecular and Biomedical Science publications

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