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|Title:||Increased intracellular T helper 1 proinflammatory cytokine production in peripheral blood, bronchoalveolar lavage and intraepithelial T cells of COPD subjects|
|Citation:||Clinical and Experimental Immunology, 2007; 150(1):22-29|
|Publisher:||Blackwell Publishing Ltd|
|G. Hodge, J. Nairn, M. Holmes, P. N. Reynolds and S. Hodge|
|Abstract:||The role of T cells in the pathophysiology of chronic obstructive pulmonary disease (COPD) is not yet certain, although varying reports have shown increases in T helper 1 (Th1) and/or Th2 cytokines in peripheral blood and bronchoalveolar lavage (BAL). No studies have examined cytokine production by intraepithelial T cells obtained by bronchial brushing (BB). Intracellular cytokine analysis of T cell subsets from peripheral blood, BAL and BB from smoker and ex-smoker COPD patients, COPD patients receiving inhaled corticosteroids and smoker and non-smoker control subjects was studied using multi-parameter flow cytometry. CD4 : CD8 inversion was noted in the peripheral blood of smoker and ex-smoker COPD groups, in BAL and BB from smoker controls and BAL of COPD smokers. There was an increase in intracellular CD8(+) T cell Th1 proinflammatory cytokines in some COPD groups in the peripheral blood and in CD8(+) T cell tumour necrosis factor (TNF)-alpha in some COPD groups and smoker controls in BAL and BB. There was an increase in proinflammatory cytokines in COPD smokers compared with ex-smokers and a decrease in COPD smokers receiving inhaled corticosteroids in the airways. There was a negative correlation between forced expiratory volume in 1 s (FEV(1)) and the percentage of BAL and intraepithelial CD8(+) T cells producing TNF-alpha. COPD patients exhibit systemic inflammation as evidenced by increased intracellular Th1 proinflammatory cytokines in blood, BAL and intraepithelial CD8(+) T cells, whereas smoker controls showed localized Th1 response in the lung only. Systemic therapeutic targeting of TNF-alpha production by CD8(+) T cells may improve morbidity in COPD patients while targeting of TNF-alpha in the lung may prevent smokers progressing to COPD.|
|Keywords:||Bronchi; Respiratory Mucosa; Th1 Cells; CD8-Positive T-Lymphocytes; Bronchoalveolar Lavage Fluid; Humans; Pulmonary Disease, Chronic Obstructive; Inflammation Mediators; Cytokines; Bronchoscopy; Forced Expiratory Volume; Lymphocyte Count; CD4 Lymphocyte Count; Adult; Aged; Middle Aged; Female; Male|
|Description:||The definitive version is available at www.blackwell-synergy.com|
|Appears in Collections:||Paediatrics publications|
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