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|Title:||Sphingosine kinase 1 is a critical component of the copper-dependent FGF1 export pathway|
|Citation:||Experimental Cell Research, 2007; 313(15):3308-3318|
|Publisher:||Academic Press Inc Elsevier Science|
|Raffaella Soldi, Anna Mandinova, Krishnan Venkataraman, Timoty Hla, Mathew Vadas, Stuart Pitson, Maria Duarte, Irene Graziani, Vihren Kolev, Doreen Kacer, Aleksandr Kirov, Thomas Maciag and Igor Prudovsky|
|Abstract:||Sphingosine kinase 1 catalyzes the formation of sphingosine-1-phosphate, a lipid mediator involved in the regulation of angiogenesis. Sphingosine kinase 1 is constitutively released from cells, even though it lacks a classical signal peptide sequence. Because copper-dependent non-classical stress-induced release of FGF1 also regulates angiogenesis, we questioned whether sphingosine kinase 1 is involved in the FGF1 release pathway. We report that (i) the coexpression of sphingosine kinase 1 with FGF1 inhibited the release of sphingosine kinase 1 at 37 degrees C; (ii) sphingosine kinase 1 was released at 42 degrees C in complex with FGF1; (iii) sphingosine kinase 1 null cells failed to release FGF1 at stress; (iv) sphingosine kinase 1 is a high affinity copper-binding protein which formed a complex with FGF1 in a cell-free system, and (v) sphingosine kinase 1 over expression rescued the release of FGF1 from inhibition by the copper chelator, tetrathiomolybdate. We propose that sphingosine kinase 1 is a component of the copper-dependent FGF1 release pathway.|
|Keywords:||Cells, Cultured; NIH 3T3 Cells; Fibroblasts; Animals; Mice, Knockout; Mice; Copper; Molybdenum; Phosphotransferases (Alcohol Group Acceptor); Fibroblast Growth Factor 1; Chelating Agents; Cloning, Molecular; Temperature; Protein Transport|
|Description:||Copyright © 2007 Elsevier Inc. All rights reserved.|
|Appears in Collections:||Molecular and Biomedical Science publications|
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