Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/45250
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Type: Journal article
Title: The chemokine receptor CCX-CKR mediates effective scavenging of CCL19 in vitro
Author: Comerford, I.
Milasta, S.
Morrow, V.
Milligan, G.
Nibbs, R.
Citation: European Journal of Immunology, 2006; 36(7):1904-1916
Publisher: Wiley-V C H Verlag GMBH
Issue Date: 2006
ISSN: 0014-2980
1521-4141
Statement of
Responsibility: 
Iain Comerford, Sandra Milasta, Valerie Morrow, Graeme Milligan and Robert Nibbs
Abstract: The chemokines CCL19, CCL21 and CCL25, by signalling through the receptors CCR7 or CCR9, play critical roles in leukocyte homing. They also bind another heptahelical surface protein, CCX-CKR. CCX-CKR cannot couple to typical chemokine receptor signalling pathways or mediate chemotaxis, and its function remains unclear. We have proposed that it controls chemokine bioavailability. Here, using transfected HEK293 cells, we have shown that both CCX-CKR and CCR7 mediate rapid CCL19 internalisation upon initial chemokine exposure. However, internalised CCL19 was more efficiently retained and degraded after uptake via CCX-CKR. More importantly, CCR7 rapidly became refractory for CCL19 uptake, but the sequestration activity of CCX-CKR was enhanced. These properties endowed CCX-CKR with an impressive ability to mediate progressive sequestration and degradation of large quantities of CCL19, and conversely, prevented CCR7-expressing cells from extensively altering their chemokine environment. These differences may be linked to the routes of endocytosis used by these receptors. CCX-CKR, unlike CCR7, was not critically dependent on beta-arrestins or clathrin-coated pits. However, over-expression of caveolin-1, which stabilises caveolae, blocked CCL19 uptake by CCX-CKR while having no impact on other chemokine receptors, including CCR7. These data predict that CCX-CKR scavenges extracellular chemokines in vivo to modify responses through CCR7.
Keywords: Cell Line
Humans
Iodine Radioisotopes
Receptors, Chemokine
Chemokines, CC
Receptors, CCR
Receptors, CCR7
Chemokine CCL19
Description: Published in European Journal of Immunology, 2006; 36 (7):1904-1916 at www.interscience.wiley.com
DOI: 10.1002/eji.200535716
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

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