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Type: Journal article
Title: Role of nuclear and cytoplasmic localization in the tumour-suppressor activity of the von Hippel-Lindau protein
Author: Lewis, M.
Roberts, B.
Citation: Oncogene, 2003; 22(26):3992-3997
Publisher: Nature Publishing Group
Issue Date: 2003
ISSN: 0950-9232
Statement of
Martin D Lewis and Ben J Roberts
Abstract: Mutations in the von Hippel-Lindau (VHL) tumour-suppressor gene result in several forms of cancer. In the present study, we investigated the role of VHL subcellular localization in its antitumour properties. We generated renal cell carcinoma (RCC) lines stably expressing either exclusively nuclear (RCC/NLS-VHL), cytoplasmic (RCC/NES-VHL) or nucleo-cytoplasmic shuttling (RCC/NES-VHL or RCC/VHL) forms of VHL and investigated several parameters linked to tumorigenesis and known to be dysregulated in VHL disease. Remarkably, although the expression of wild-type VHL is largely cytoplasmic, all of the antitumour properties of VHL tested could be reconstituted by expressing exclusively nuclear VHL.
Keywords: Tumor Cells, Cultured
Cell Nucleus
Carcinoma, Renal Cell
Kidney Neoplasms
Ubiquitin-Protein Ligases
Bacterial Proteins
Monosaccharide Transport Proteins
Luminescent Proteins
Integrin alpha5
Tumor Suppressor Proteins
Recombinant Proteins
Transcription Factors
Drug Combinations
Microscopy, Fluorescence
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Neoplastic
Amino Acid Sequence
Protein Structure, Tertiary
Genetic Vectors
Molecular Sequence Data
Glucose Transporter Type 1
Von Hippel-Lindau Tumor Suppressor Protein
Hypoxia-Inducible Factor 1, alpha Subunit
Rights: © 2003 Nature Publishing Group. All rights reserved.
DOI: 10.1038/sj.onc.1206683
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

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