Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Primary defect in UVB-induced systemic immunomodulation does not relate to immature or functionally impaired APCs in regional lymph nodes
Author: Gorman, S.
Tan, J.
Thomas, J.
Townley, S.
Stumbles, P.
Finlay-Jones, J.
Hart, P.
Citation: Journal of Immunology, 2005; 174(11):6677-6685
Publisher: Amer Assoc Immunologists
Issue Date: 2005
ISSN: 0022-1767
Statement of
Shelley Gorman, Jamie W.-Y. Tan, Jennifer A. Thomas, Scott L. Townley, Philip A. Stumbles, John J. Finlay-Jones and Prue H. Hart
Abstract: UVB irradiation of the shaved dorsal skin of mice can cause both local and systemic suppression of contact hypersensitivity responses; the former demonstrated by administration of the sensitizing Ag/hapten to the irradiated site and the latter by its administration at least 72 h later to distal unirradiated sites. The immunological basis of systemic immunomodulation is not clear. When haptens (trinitrochlorobenzene, FITC) were administered to the shaved ventral skin 4 days after irradiation (8 kJ/m(2)) to the shaved dorsum of BALB/c mice, CD11c(+)/FITC(+) cells in the skin-draining lymph nodes from control and irradiated mice produced on a per cell basis similar levels of IL-12 and PGE(2) were phenotypically mature and efficient at presenting FITC to lymphocytes from FITC-sensitized mice. Ag presentation by FACS-sorted CD11c(+) lymph node cells isolated 4 days after UVB irradiation was as efficient as were cells from unirradiated mice at presentation in vitro of an OVA peptide (OVA(323-339)) to CD4(+) cells from OVA-TCR-transgenic DO11.10 mice. Further, IFN-gamma levels were increased in the cultures containing CD11c(+) cells from UVB-irradiated mice, suggesting that inflammation may precede downstream immunosuppression. These results suggest that the primary cause of reduced contact hypersensitivity responses in mice in which UV irradiation and the sensitizing Ag are applied to different sites several days apart must originate from cells other than CD11c(+) APCs that directly or by production of soluble mediators (IL-12, PGE(2)) affect cellular responses in the nodes of UVB-irradiated mice.
Keywords: Lymph Nodes
Antigen-Presenting Cells
Dendritic Cells
Mice, Inbred BALB C
Mice, Transgenic
Dermatitis, Contact
Picryl Chloride
Immunologic Factors
Coculture Techniques
Administration, Topical
Ultraviolet Rays
Cell Differentiation
Cell Movement
Antigen Presentation
Amino Acid Sequence
Molecular Sequence Data
CD11c Antigen
Description: Copyright © 2005 by The American Association of Immunologists, Inc.
DOI: 10.4049/jimmunol.174.11.6677
Published version:
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.