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Type: Journal article
Title: Primary human osteoblasts grow into porous tantalum and maintain an osteoblastic phenotype
Author: Welldon, K.
Atkins, G.
Howie, D.
Finlay, D.
Citation: Journal of Biomedical Materials Research. Part A, 2008; 84A(3):691-701
Publisher: John Wiley & Sons, Inc.
Issue Date: 2008
ISSN: 1549-3296
Statement of
Katie J. Welldon, Gerald J. Atkins, Donald W. Howie and David M. Findlay
Abstract: Porous tantalum (Ta) has found application in orthopedics, although the interaction of human osteoblasts (HOB) with this material has not been reported. The aim of this study was to investigate the interaction of primary HOB with porous tantalum, using 5-mm thick discs of porous tantalum. Comparison was made with discs of solid tantalum and tissue culture plastic. Confocal microscopy was used to investigate the attachment and growth of cells on porous Ta, and showed that HOB attached successfully to the metal "trabeculae," underwent extensive cell division, and penetrated into the Ta pores. The maturation of HOB on porous Ta was determined in terms of cell expression of the osteoblast phenotypic markers, STRO-1, and alkaline phosphatase. Despite some donor-dependent variation in STRO-1/AlkPhos expression, growth of cells grown on porous Ta either promoted, or did not impede, the maturation of HOB. In addition, the expression of key osteoblastic genes was investigated after 14 days of culture. The relative levels of mRNA encoding osteocalcin, osteopontin and receptor activator of NFkappaB ligand (RANKL) was not different between porous or solid Ta or plastic, although these genes were expressed differently by cells of different donors. However, bone sialoprotein and type I collagen mRNA species showed a decreased expression on porous Ta compared with expression on plastic. No substrate-dependent differences were seen in the extent of in vitro mineralization by HOB. These results indicate that porous Ta is a good substrate for the attachment, growth, and differentiated function of HOB.
Keywords: Cells, Cultured; Osteoblasts; Humans; Tantalum; Biocompatible Materials; Cell Adhesion; Phenotype; Porosity; Aged; Aged, 80 and over; Middle Aged; Female; Male
Description: Published in Journal of Biomedical Materials Research. Part A, 2008; 84A (3):691-701 at
RMID: 0020080240
DOI: 10.1002/jbm.a.31336
Appears in Collections:Orthopaedics and Trauma publications

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