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dc.contributor.authorNash, R.en
dc.contributor.authorNeves, L.en
dc.contributor.authorFaast, R.en
dc.contributor.authorPierce, M.en
dc.contributor.authorDalton, S.en
dc.date.issued2007en
dc.identifier.citationStem Cells, 2007; 25(4):974-982en
dc.identifier.issn1066-5099en
dc.identifier.issn1549-4918en
dc.identifier.urihttp://hdl.handle.net/2440/45512-
dc.descriptionCopyright © 2007 by AlphaMed Press.en
dc.description.abstractCell surface markers are key tools that are frequently used to characterize and separate mixed cell populations. Existing cell surface markers used to define murine embryonic stem cells (mESCs) such as stage-specific embryonic antigen 1 (SSEA1), Forssman antigen (FA), alkaline phosphatase (AP), and CD9 are limiting, however, because they do not unambiguously define the pluripotent state and are not reliable indicators of differentiation commitment. To identify glycan cell surface markers that would circumvent this problem, we used a panel of 18 lectins to identify epitopes specifically elevated on the surface of mESCs, which, during differentiation, decrease with kinetics that precede currently used markers such as CD9, SSEA1, FA, and AP. The anticipated outcome of this analysis was to identify glycans that have utility as reliable mESC markers and high-resolution readouts for early differentiation commitment. Here, we show that the lectin Dolichos biflorus agglutinin (DBA) recognizes -N-acetylgalactosamine (GalNAc) cell surface epitopes on mESCs (CD9high SSEA1high APhigh DBAhigh). These glycan epitopes decline markedly in cells undergoing the first definable step of differentiation, the transition from mESCs to primitive ectoderm (CD9high SSEA1high APhigh DBAlow). Loss of GalNAc epitopes is, therefore, the earliest cell surface change that can be assigned to differentiating cells, and the only cell surface marker known to be tightly associated with the pluripotent state. The lectin DBA is, therefore, a useful tool to characterize mESC cultures by nondestructive approaches, an indicator of differentiation commitment, and a predictor of developmental potency.en
dc.description.statementofresponsibilityRodney Nash, Lori Neves, Renate Faast, Michael Pierce and Stephen Daltonen
dc.language.isoenen
dc.publisherAlphamed Pressen
dc.subjectEmbryonic stem cells; Primitive ectoderm; Lectin; Differentiationen
dc.titleThe lectin dolichos biflorus agglutinin recognizes glycan epitopes on the surface of murine embryonic stem cells: A new tool for characterizing pluripotent cells and early differentiationen
dc.typeJournal articleen
dc.provenanceFirst published online December 14, 2006en
dc.identifier.doi10.1634/stemcells.2006-0224en
pubs.publication-statusPublisheden
Appears in Collections:Molecular and Biomedical Science publications

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