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Type: Journal article
Title: An ALMT1 gene cluster controlling aluminum tolerance at the Alt4 locus of rye (Secale cereale L.)
Author: Collins, N.
Shirley, N.
Saeed, M.
Pallotta, M.
Gustafson, J.
Citation: Genetics: a periodical record of investigations bearing on heredity and variation, 2008; 179(1):669-682
Publisher: Genetics
Issue Date: 2008
ISSN: 0016-6731
Statement of
N. C. Collins, N. J. Shirley, M. Saeed, M. Pallotta and J. P. Gustafson
Abstract: Aluminum toxicity is a major problem in agriculture worldwide. Among the cultivated Triticeae, rye (Secale cereale L.) is one of the most Al tolerant and represents an important potential source of Al tolerance for improvement of wheat. The Alt4 Al-tolerance locus of rye contains a cluster of genes homologous to the single-copy Al-activated malate transporter (TaALMT1) Al-tolerance gene of wheat. Tolerant (M39A-1-6) and intolerant (M77A-1) rye haplotypes contain five and two genes, respectively, of which two (ScALMT1-M39.1 and ScALMT1-M39.2) and one (ScALMT1-M77.1) are highly expressed in the root tip, typically the main site of plant Al tolerance/susceptibility. All three transcripts are upregulated by exposure to Al. High-resolution genetic mapping identified two resistant lines resulting from recombination within the gene cluster. These recombinants exclude all genes flanking the gene cluster as candidates for controlling Alt4 tolerance, including a homolog of the barley HvMATE Al-tolerance gene. In the recombinants, one hybrid gene containing a chimeric open reading frame and the ScALMT1-M39.1 gene each appeared to be sufficient to provide full tolerance. mRNA splice variation was observed for two of the rye ALMT1 genes and in one case, was correlated with a ~400-bp insertion in an intron.
Keywords: Aluminum
Organic Anion Transporters
Blotting, Southern
Chromosome Mapping
Sequence Analysis, DNA
Base Sequence
Drug Tolerance
Multigene Family
Models, Genetic
Molecular Sequence Data
Description: Copyright © 2008 by the Genetics Society of America.
DOI: 10.1534/genetics.107.083451
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Appears in Collections:Agriculture, Food and Wine publications
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