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Type: Journal article
Title: A novel tetrafunctional reagent for simultaneous intramolecular and intermolecular cross-linking of bovine hemoglobin
Author: Yun, Q.
He, M.
Xing, W.
Bi, J.
Ma, G.
Su, Z.
Citation: Biotechnology Letters, 2004; 26(17):1359-1363
Publisher: Kluwer Academic Publ
Issue Date: 2004
ISSN: 0141-5492
Statement of
Qiang Yun, Minglei He, Wenchao Xing, Jingxiu Bi, Guanghui Ma & Zhiguo Su
Abstract: A novel tetrafunctional reagent, α, γ, α, γ-tetra-succinimidyl-hexanediamide-di-glutamate ester (HDG(OSu)4), was successfully synthesized, and a well-defined cross-linked bovine hemoglobin (mainly 128 kDa) was prepared with this reagent. Due to the spatial structure of this cross-linking reagent, the intramolecular and intermolecular cross-linking of bovine hemoglobin was formed simultaneously in one reaction. Although the cross-linked bovine hemoglobin showed a slight decrease in half-saturated O2 pressure value (P50, from 28.1 mm Hg to 21.7 mm Hg) and Hill coefficient (from 2.5 to 2), due to the cross-linkage, it still performed well for O2 delivery.
Keywords: blood substitute
bovine hemoglobin
cross-linking reagent
Description: The definitive version may be found at
DOI: 10.1023/B:BILE.0000045633.36744.0d
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Chemical Engineering publications

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