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|Title:||A novel tetrafunctional reagent for simultaneous intramolecular and intermolecular cross-linking of bovine hemoglobin|
|Citation:||Biotechnology Letters, 2004; 26(17):1359-1363|
|Publisher:||Kluwer Academic Publ|
|Qiang Yun, Minglei He, Wenchao Xing, Jingxiu Bi, Guanghui Ma & Zhiguo Su|
|Abstract:||A novel tetrafunctional reagent, α, γ, α, γ-tetra-succinimidyl-hexanediamide-di-glutamate ester (HDG(OSu)4), was successfully synthesized, and a well-defined cross-linked bovine hemoglobin (mainly 128 kDa) was prepared with this reagent. Due to the spatial structure of this cross-linking reagent, the intramolecular and intermolecular cross-linking of bovine hemoglobin was formed simultaneously in one reaction. Although the cross-linked bovine hemoglobin showed a slight decrease in half-saturated O2 pressure value (P50, from 28.1 mm Hg to 21.7 mm Hg) and Hill coefficient (from 2.5 to 2), due to the cross-linkage, it still performed well for O2 delivery.|
|Description:||The definitive version may be found at http://www.springerlink.com/|
|Appears in Collections:||Aurora harvest 6|
Chemical Engineering publications
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