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Type: Journal article
Title: Human c-fos promoter mediates high-level, inducible expression in various mammalian cell lines
Author: Bi, J.
Buhr, P.
Zeng, A.
Wirth, M.
Citation: Biotechnology and Bioengineering, 2003; 81(7):848-854
Publisher: John Wiley & Sons Inc
Issue Date: 2003
ISSN: 0006-3592
Abstract: The promoter activity of the human c-fos and human cytomegalovirus (CMV) immediate early promoter was compared in transient and stable transfection experiments with six cell lines of mouse, human, and hamster origin which are all of commercial importance. The c-fos promoter was 1.8-5.6-fold stronger than the CMV promoter in BHK-A, BHK-B, CHO-DHFR(-), and mouse NIH-3T3 in stable transfectants and less effective in mouse myeloma or human 293 cells, suggesting a new transcriptional control element for high-level expression and protein production in mammalian cells. The induction profiles determined in the presence and absence of serum are dependent on the cell line used. Induction levels of up to 8-fold could be achieved in preselected cell pools.
Keywords: human c-fos promoter; gene expression; mammalian cell
Description: © 2003 Wiley Periodicals, Inc.
RMID: 0020081599
DOI: 10.1002/bit.10530
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Appears in Collections:Chemical Engineering publications

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