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Type: Journal article
Title: Regulation of 15-hydroxyprostaglandin dehydrogenase (PGDH) gene activity messenger ribonucleic acid processing and protein abundance in the human chorion in late gestation and labor
Author: Johnson, R.
Mitchell, C.
Clifton, V.
Zakar, T.
Citation: Journal of Clinical Endocrinology and Metabolism, 2004; 89(11):5639-5648
Publisher: Endocrine Society
Issue Date: 2004
ISSN: 0021-972X
Statement of
Renée F. Johnson, Carolyn M. Mitchell, Vicki Clifton and Tamas Zakar
Abstract: The prostaglandin (PG)-inactivating enzyme 15-hydroxyprostaglandin dehydrogenase (PGDH) is highly expressed in the chorion leave. To assess the involvement of PGDH in the regulation of intrauterine PG levels, we have determined the mechanisms that control chorionic PGDH expression in women at term and preterm labor. PGDH gene activity decreased at term and during normal labor. PGDH mRNA abundance also decreased at term due to changing splice variant distribution. Gene activity predicted PGDH mRNA abundance preterm and after normal labor, but not at term before labor. PGDH mRNA decayed rapidly in cultured tissues and was stabilized by transcriptional arrest. PGDH protein levels varied without being significantly different between the patient groups. PGDH mRNA levels predicted PGDH protein levels at term, but not preterm and after labor. PGDH gene activity, mRNA variant, and immunoreactive protein levels were not different between the preterm labor and preterm not in labor groups. Thus, PGDH mRNA is transiently down-regulated before term labor by a posttranscriptional mechanism(s). Protein turnover controls PGDH protein abundance at preterm and after normal labor. At term, PGDH protein levels become dependent on the rapidly turning over PGDH mRNA. This may allow rapid changes in PGDH protein abundance and uterotonic PG concentrations promoting labor.
Keywords: Chorion
Hydroxyprostaglandin Dehydrogenases
RNA, Messenger
Labor, Obstetric
Description: Copyright © 2004 by The Endocrine Society
DOI: 10.1210/jc.2004-0540
Published version:
Appears in Collections:Aurora harvest 6
Obstetrics and Gynaecology publications

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