Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/46753
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Characterization of human fetal cord blood steroid profiles in relation to fetal sex and mode of delivery using temperature-dependent inclusion chromatography and principal component analysis (PCA)
Author: Clifton, V.
Bisits, A.
Zarzycki, P.
Citation: Journal of Chromatography. B, Analytical Techniques in the Biomedical and Life Sciences, 2007; 855(2):249-254
Publisher: Elsevier Science BV
Issue Date: 2007
ISSN: 1570-0232
1873-376X
Statement of
Responsibility: 
Vicki L. Clifton, Andrew Bisits and Paweł K. Zarzycki
Abstract: In the present work, human male and female fetal cord blood samples were purified, selectively extracted and separated to examine a fraction of steroids ranging from polar estetrol to relatively non-polar progesterone using solid phase extraction based on C-18 tubes and β-cyclodextrin driven temperature dependent inclusion chromatography. Resulting UV diode array chromatographic patterns revealed the presence of 27 peaks. Chromatographic patterns of UV detected steroids were analyzed using principal components analysis which revealed differences between male/female and labour/not-in-labour clusters. Quantitative analysis of nine identified steroids including: estetrol, 17β-estradiol, estrone, estriol, cortisol, cortisone, progesterone, 20greek small letter alpha-hydroxyprogesterone and 17greek small letter alpha-hydroxyprogesterone were not significantly different between males and females. Significant differences between male and female fetuses were related to as yet unidentified compounds. Four peaks were significantly different with labour which corresponded with cortisol, cortisone and two unidentified compounds. This protocol may distinguish significant differences between clinical groups that are not readily identifiable using univariate measurements of single steroids or different low molecular mass biomarkers. Moreover, we have provided new evidence that despite the absence of testosterone there are number of steroids and low molecular mass compounds that differ between male and female fetuses.
Keywords: Human pregnancy; Fetal sex; Labour; Cord blood; Chromatography; Principle component analysis
Description: Copyright © 2007 Elsevier B.V. All rights reserved.
RMID: 0020081042
DOI: 10.1016/j.jchromb.2007.05.041
Description (link): http://www.elsevier.com/wps/find/journaldescription.cws_home/643040/description#description
Appears in Collections:Paediatrics publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.