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https://hdl.handle.net/2440/46963
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Type: | Journal article |
Title: | Characterization of the PGK2 associated microsatellite S0719 on SSC7 suitable for parentage and QTL diagnosis |
Author: | Chen, K. Knorr, C. Bornemann-Kolatzki, K. Huang, L. Rohrer, G. Brenig, B. |
Citation: | Animal Biotechnology, 2006; 17(1):43-49 |
Publisher: | Marcel Dekker Inc |
Issue Date: | 2006 |
ISSN: | 1049-5398 1532-2378 |
Abstract: | We isolated and characterized the highly polymorphic tetra-nucleotide microsatellite S0719 on SSC7q14-q15 adjacent to the porcine testis-specific phosphoglycerate kinase 2 (PGK2) gene and assigned it to the USDA-MARC linkage map on SSC7 position 77.5 cM closely linked to markers SW859 (76.3 cM) and SWR2036 (79.0 cM). In a panel of 344 individuals representing 11 pig breeds (European, Chinese, and North American), a total of 32 alleles were observed, and the overall breeds' calculated PIC (polymorphism information content), HE (heterozygosity), and NE (effective allele number) were 0.94, 0.94, and 16.41. Breed-specific PIC and HE ranged from 0.66 to 0.87, whereas NE was as low as 2.95 and as high as 7.96. Considering the high allelic variation of S0719 within and among pig breeds (79% of the genotyped animals were heterozygous), the marker is useful for individual animal identification and parentage determination. Finally, S0719 is also a valuable STS marker for fine-mapping QTL on SSC7 as position 77.5 cM is located in 25 QTL intervals |
Keywords: | PIG S0719 Microsatellite PGK2 |
DOI: | 10.1080/10495390500461005 |
Published version: | http://dx.doi.org/10.1080/10495390500461005 |
Appears in Collections: | Aurora harvest 6 Earth and Environmental Sciences publications Environment Institute publications |
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