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|Title:||The endothelium-derived hyperpolarizing factor H2O2 promotes metal-ion efflux in aortic endothelial cells: Elemental mapping by a hard X-ray microprobe|
|Citation:||Biochemistry, 2006; 45(41):12500-12509|
|Publisher:||Amer Chemical Soc|
|Paul K. Witting, Hugh H. Harris, Benjamin S. Rayner, Jade B. Aitken, Carolyn T. Dillon, Roland Stocker, Barry Lai, Zhonghou Cai, and Peter A. Lay|
|Abstract:||Hydrogen peroxide (H(2)O(2)) is a physiologic oxidant implicated in vascular cell signaling, although little is known about the biochemical consequences of its reaction with endothelial cells. Submicrometer-resolution hard X-ray elemental mapping of cultured porcine aortic endothelial cells (PAEC) has provided data on the global changes for intracellular elemental density within PAEC and indicates an efflux of metal ions and phosphorus from the cytoplasm after H(2)O(2) treatment. The synchrotron-radiation-induced X-ray emission experiments (SRIXE) show that H(2)O(2)-treated cells are irregularly shaped and exhibit blebbing indicative of increased permeability due to the damaged membrane. The SRIXE results suggest that H(2)O(2)-induced damage is largely restricted to the cell membrane as judged by the changes to membrane and cytoplasmic components rather than the cell nucleus. The SRIXE data also provide a mechanism for cell detoxification as the metal-ion efflux resulting from the initial H(2)O(2)-mediated changes to cell membrane potentially limits intracellular metal-mediated redox processes through Fenton-like chemistry. They may also explain the increased levels of these ions in atherosclerotic plaques, regardless of whether they are involved in plaque formation. Finally, the SRIXE data support the notion that cultured endothelial cells exposed to H(2)O(2) respond with enhanced cellular metal-ion efflux into the extracellular space.|
|Keywords:||Cells, Cultured; Endothelial Cells; Animals; Swine; Hydrogen Peroxide; Metals; Biological Factors; Electron Probe Microanalysis; Calcium Signaling; Ion Transport; Synchrotrons; Models, Biological|
|Description:||Copyright © 2006 American Chemical Society|
|Appears in Collections:||Chemistry and Physics publications|
Environment Institute publications
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