Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/47273
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dc.contributor.authorFalk, T.en
dc.contributor.authorStrazdas, L.en
dc.contributor.authorBorders, R.en
dc.contributor.authorKilani, R.en
dc.contributor.authorYool, A.en
dc.contributor.authorSherman, S.en
dc.date.issued2001en
dc.identifier.citationElectronic Journal of Biotechnology, 2001; 4(1):33-45en
dc.identifier.issn0717-3458en
dc.identifier.issn0717-3458en
dc.identifier.urihttp://hdl.handle.net/2440/47273-
dc.description.abstractHigh-density cultures of mammalian neurons offer a model system for studies of brain development, but the morphological features of individual neurons is difficult to ascertain. We show that a herpes virus vector expressing a bioluminescent protein allows detailed morphometric analyses of living neurons in complex culture environments. Expression of enhanced green fluorescent protein (eGFP) was constitutively driven in neurons using the herpes simplex virus amplicon system. This system allowed us to make novel observations regarding development in high-density cultures from rat hippocampus and cerebellum. After the phase of initial neurite outgrowth, maturing neurons continue to show rapid remodeling of the neurite branches (0.79 ± 0.11 mm/h per neurite; mean ± SEM, n=8), and displacement of the soma within the neurite arbor (1.35 ± 0.74 mm/h). These results demonstrate that a substantial capacity for morphological plasticity persists in maturing mammalian CNS neurons after cessation of net neurite outgrowth in early development.en
dc.description.statementofresponsibilityFalk, Torsten; Strazdas, Lori A.; Borders, Rebecca S.; Kilani, Ramsey K.; Yool, Andrea J.; Sherman, Scott J.en
dc.language.isoenen
dc.publisherPontificia Universidad Católica de Valparaísoen
dc.rights© 2001 by Universidad Católica de Valparaísoen
dc.source.urihttp://www.ejbiotechnology.info/content/vol4/issue1/full/5/bip/index.htmlen
dc.subjectCerebellum; green fluorescent protein; hippocampus; plasticity; Purkinje neuronen
dc.titleA herpes simplex viral vector expressing green fluorescent protein can be used to visualize morphological changes in high-density neuronal cultureen
dc.typeJournal articleen
pubs.publication-statusPublisheden
dc.identifier.orcidYool, A. [0000-0003-1283-585X]en
Appears in Collections:Molecular and Biomedical Science publications

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