Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/47316
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Effects of female steroid hormones on A-type K⁺ currents in murine colon
Other Titles: Effects of female steroid hormones on A-type K(+) currents in murine colon
Author: Spencer, E.
McCloskey, C.
O'Kane, N.
Sanders, K.
Koh, S.
Citation: The Journal of Physiology, 2006; 573(2):453-468
Publisher: Blackwell Publishing Ltd
Issue Date: 2006
ISSN: 0022-3751
1469-7793
Statement of
Responsibility: 
Elizabeth A. H. Beckett, Conor McCloskey, Neil O'Kane, Kenton M. Sanders and Sang Don Koh
Abstract: Idiopathic constipation is higher in women of reproductive age than postmenopausal women or men, suggesting that female steroid hormones influence gastrointestinal motility. How female hormones affect motility is unclear. Colonic motility is regulated by ion channels in colonic myocytes. Voltage-dependent K+ channels serve to set the excitability of colonic muscles. We investigated regulation of Kv4.3 channel expression in response to acute or chronic changes in female hormones. Patch clamp experiments and quantitative PCR were used to compare outward currents and transcript expression in colonic myocytes from male, non-pregnant, pregnant and ovariectomized mice. Groups of ovariectomized mice received injections of oestrogen or progesterone to investigate the effects of hormone replacement. The capacitance of colonic myocytes from non-pregnant females was larger than in males. Net outward current density in male and ovariectomized mice was higher than in non-pregnant females and oestrogen-treated ovariectomized mice. Current densities in late pregnancy were lower than in female controls. Progesterone had no effect on outward currents. A-type currents were decreased in non-pregnant females compared with ovariectomized mice, and were further decreased by pregnancy or oestrogen replacement. Kv4.3 transcripts did not differ significantly between groups; however, expression of the potassium channel interacting protein KChIP1 was elevated in ovariectomized mice compared with female controls and oestrogen-treated ovariectomized mice. Delayed rectifier currents were not affected by oestrogen. In the mouse colon, oestrogen suppresses A-type currents, which are important for regulating excitability. These observations suggest a possible link between female hormones and altered colonic motility associated with menses, pregnancy and menopause.
Keywords: Colon
Animals
Mice, Inbred BALB C
Mice
Gonadal Steroid Hormones
Ovariectomy
Pregnancy
Female
Male
Kv Channel-Interacting Proteins
Rights: Copyright © 2006 The Physiological Society.
DOI: 10.1113/jphysiol.2006.107375
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.