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Type: Journal article
Title: Polycomblike PHD Fingers Mediate Conserved Interaction with Enhancer of Zeste Protein
Author: O'Connell, S.
Wang, L.
Robert, S.
Jones, C.
Saint, R.
Jones, R.
Citation: Journal of Biological Chemistry, 2001; 276(46):43065-43073
Publisher: Amer Soc Biochemistry Molecular Biology Inc
Issue Date: 2001
ISSN: 0021-9258
Organisation: Centre for the Molecular Genetics of Development
Abstract: The products of Polycomb group (PcG) genes are required for the epigenetic repression of a number of important developmental regulatory genes, including homeotic genes. Enhancer of zeste (E(Z)) is a Drosophila PcG protein that previously has been shown to bind directly to another PcG protein, Extra Sex Combs (ESC), and is present along with ESC in a 600-kDa complex in Drosophila embryos. Using yeast two-hybrid and in vitro binding assays, we show that E(Z) binds directly to another PcG protein, Polycomblike (PCL). PCL·E(Z) interaction is shown to be mediated by the plant homeodomain (PHD) fingers domain of PCL, providing evidence that this motif can act as an independent protein interaction domain. An association was also observed between PHF1 and EZH2, human homologs of PCL and E(Z), respectively, demonstrating the evolutionary conservation of this interaction. E(Z) was found to not interact with the PHD domains of three Drosophila trithorax group (trxG) proteins, which function to maintain the transcriptional activity of homeotic genes, providing evidence for the specificity of the interaction of E(Z) with the PCL PHD domain. Coimmunoprecipitation and gel filtration experiments demonstrate in vivo association of PCL with E(Z) and ESC in Drosophila embryos. We discuss the implications of PCL association with ESC·E(Z) complexes and the possibility that PCL may either be a subunit of a subset of ESC·E(Z) complexes or a subunit of a separate complex that interacts with ESC·E(Z) complexes.
Keywords: Animals
Drosophila Proteins
Nuclear Proteins
Repressor Proteins
Blotting, Western
Precipitin Tests
Chromatography, Gel
Two-Hybrid System Techniques
Mutagenesis, Site-Directed
Transcription, Genetic
Amino Acid Sequence
Amino Acid Motifs
Conserved Sequence
Zinc Fingers
Protein Structure, Tertiary
Protein Binding
Sequence Homology, Amino Acid
Point Mutation
Molecular Sequence Data
Polycomb Repressive Complex 2
DOI: 10.1074/jbc.M104294200
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Appears in Collections:Aurora harvest
Centre for the Molecular Genetics of Development publications

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