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https://hdl.handle.net/2440/47843
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Type: | Journal article |
Title: | Tum/RacGAP50C provides a critical link between anaphase microtubules and the assembly of the contractile ring in Drosophila melanogaster |
Author: | Zavortink, M. Contreras, N. Addy, T. Bejsovec, A. Saint, R. |
Citation: | Journal of Cell Science, 2005; 118(22):5381-5392 |
Publisher: | Company of Biologists Ltd |
Issue Date: | 2005 |
ISSN: | 0021-9533 1477-9137 |
Organisation: | Centre for the Molecular Genetics of Development |
Abstract: | A central question in understanding cytokinesis is how the cleavage plane is positioned. Although the positioning signal is likely to be transmitted via the anaphase microtubule array to the cell cortex, exactly how the microtubule array determines the site of contractile ring formation remains unresolved. By analysing tum/RacGAP50C mutant Drosophila embryos we show that cells lacking Tum do not form furrows and fail to localise the key cytokinetic components Pebble (a RhoGEF), Aurora B kinase, Diaphanous, Pav-KLP and Anillin. The GAP activity of Tum is required for cytokinesis: in its absence cytokinesis fails early even though Tum is present on microtubules at the cell equator where the furrow should form. Disruption of the Pebble-interacting domain leaves Tum localised to the cell equator on cortically associated microtubules, again with no evidence of furrowing. These data support a model in which Tum/RacGAP, via its interaction with Pbl, provides a critical link between the anaphase microtubule spindle and cytokinetic furrow formation in Drosophila cells. |
Keywords: | Microtubules Epithelial Cells Ectoderm Animals Drosophila melanogaster GTPase-Activating Proteins Guanine Nucleotide Exchange Factors Contractile Proteins Microtubule-Associated Proteins Drosophila Proteins Anaphase Telophase Cytokinesis Embryonic Development Mutation |
DOI: | 10.1242/jcs.02652 |
Published version: | http://jcs.biologists.org/cgi/content/abstract/118/22/5381 |
Appears in Collections: | Aurora harvest 6 Centre for the Molecular Genetics of Development publications |
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