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Type: Journal article
Title: Negative ion fragmentations of deprotonated peptides: backbone cleavages directed through both Asp and Glu
Author: Brinkworth, C.
Dua, S.
Mc Anoy, A.
Bowie, J.
Citation: Rapid Communications in Mass Spectrometry, 2001; 15(20):1965-1973
Publisher: John Wiley & Sons Ltd
Issue Date: 2001
ISSN: 0951-4198
Statement of
Craig S. Brinkworth, Suresh Dua, Andrew M. McAnoy, John H. Bowie
Abstract: The collision-induced spectra of [M - H](-) ions of a variety of natural and synthetic amphibian peptides containing Asp and/or Glu exhibit characteristic gamma backbone cleavage ions that identify the positions of these residues in the peptide. A theoretical study suggests that the Glu cleavage involves an S(N)i reaction of the carboxylate anion from the Glu alpha side chain to form a deprotonated cyclic lactone. The presence of either Asp or Glu or other residues that effect pronounced side-chain cleavages (e.g. Ser or Thr) results in the normal alpha and beta backbone cleavages being reduced in comparison to those cleavages which originate from side chains.
Keywords: Animals
Aspartic Acid
Glutamic Acid
Spectrometry, Mass, Fast Atom Bombardment
Amino Acid Sequence
Elementary Particle Interactions
Molecular Sequence Data
Mass Spectrometry
Description: The definitive version may be found at
Provenance: Published Online: 1 Oct 2001
DOI: 10.1002/rcm.457
Grant ID: ARC
Published version:
Appears in Collections:Aurora harvest 6
Chemistry publications

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