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|Title:||Receptor tyrosine kinase signaling regulates different modes of Groucho-dependent control of Dorsal|
|Citation:||Current Biology, 2000; 10(1):51-54|
|Organisation:||Centre for the Molecular Genetics of Development|
|Häder T, Wainwright D, Shandala T, Saint R, Taubert H, Brönner G and Jäckle H.|
|Abstract:||Transcriptional control of the Drosophila terminal gap gene huckebein (hkb) depends on Torso (Tor) receptor tyrosine kinase (RTK) signaling and the Rel/NFB homolog Dorsal (Dl) . Dl acts as an intrinsic transcriptional activator in the ventral region of the embryo, but under certain conditions, such as when it is associated with the non-DNA-binding co-repressor Groucho (Gro), it is converted into a repressor . Gro is recruited to the enhancer element in the vicinity of Dl by sequence-specific transcription factors such as Dead Ringer (Dri) . We examined the interplay between Dl, Gro and Dri on the hkb enhancer and show that when acting over a distance, Gro abolishes rather than converts Dl activator function. Reducing the distance between Dl- and Dri-binding sites, however, switches Dl into a Gro-dependent repressor that overrides activation of transcription. Both of the distance-dependent regulatory options of Gro - quenching and silencing of transcription- are inhibited by RTK signaling. These data describe a newly identified mode of function for Gro when acting in concert with Dl. RTK signaling provides a way of modulating Dl function by interfering either with Gro activity or with Dri-dependent recruitment of Gro to the enhancer.|
|Keywords:||Mesoderm; Animals; Animals, Genetically Modified; Drosophila melanogaster; Receptor Protein-Tyrosine Kinases; DNA-Binding Proteins; Insect Proteins; Drosophila Proteins; Nuclear Proteins; Phosphoproteins; Recombinant Fusion Proteins; Transcription Factors; Repressor Proteins; Mutagenesis, Site-Directed; Signal Transduction; Transcription, Genetic; Gene Expression Regulation, Developmental; Gene Silencing; Sequence Deletion; Base Sequence; Regulatory Sequences, Nucleic Acid; Morphogenesis; Molecular Sequence Data; Basic Helix-Loop-Helix Transcription Factors|
|Appears in Collections:||Molecular and Biomedical Science publications|
Centre for the Molecular Genetics of Development publications
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