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Type: Journal article
Title: Enrichment of multiphosphorylated peptides by immobilized metal affinity chromatography using Ga(III)- and Fe(III)-complexes
Author: Sykora, C.
Hoffmann, R.
Hoffmann, P.
Citation: Protein and Peptide Letters: international journal for rapid publication of short papers in protein and peptide science, 2007; 14(5):489-496
Publisher: Bentham Science Publ Ltd
Issue Date: 2007
ISSN: 0929-8665
Abstract: The detection and identification of O-phosphorylation sites in proteins with mass spectrometry remains a challenge. A common approach to analyse these modifications is to enrich phosphopeptides by immobilized metal affinity chromatography (IMAC) prior to mass spectrometric analysis. In this study two commercially available IMAC kits based on Fe(III)-ions immobilized on magnetic beads and Ga(III)-ions immobilized on a chelate-resin, have been investigated and the binding efficiency of peptide mixtures containing non-phosphorylated, singly, doubly and triply phosphorylated peptides have been tested.
Keywords: Humans
Ferric Compounds
tau Proteins
Reagent Kits, Diagnostic
Chromatography, Affinity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
DOI: 10.2174/092986607780782849
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Appears in Collections:Aurora harvest 5
Molecular and Biomedical Science publications

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