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dc.contributor.authorSasseville, M.-
dc.contributor.authorGagnon, M.-
dc.contributor.authorGuillemette, C.-
dc.contributor.authorSullivan, R.-
dc.contributor.authorGilchrist, R.-
dc.contributor.authorRichard, F.-
dc.identifier.citationMolecular Endocrinology, 2009; 23(5):700-710-
dc.descriptionCopyright © 2009 by The Endocrine Society-
dc.description.abstractGap-junctional communication (GJC) plays a central role in oocyte growth. However, little is known about the regulation of connexin 43 (Cx43)-based gap-junction channels in cumulus-oocyte complexes (COCs) during in vitro maturation. We show that rupture of COCs from mural granulosa cells up-regulates Cx43-mediated GJC and that gonadotropins signal GJC breakdown by recruiting Cx43 to lipid rafts when oocyte meiosis resumes. Oocyte calcein uptake through gap junctions increases during early in vitro oocyte maturation and remains high until 18 h, when it falls simultaneously with the oocyte germinal vesicle breakdown. Immunodetection of Cx43 and fluorescence recovery after photobleaching assays revealed that the increase of GJC is independent of gonadotropins but requires RNA transcription, RNA polyadenylation, and translation. GJC rupture, in contrast, is achieved by a gonadotropin-dependent mechanism involving recruitment of Cx43 to clustered lipid rafts. These results show that GJC up-regulation in COCs in in vitro culture is independent of gonadotropins and transcriptionally regulated. However, GJC breakdown is gonadotropin dependent and mediated by the clustering of Cx43 in lipid raft microdomains. In conclusion, this study supports a functional role of lipid raft clustering of Cx43 in GJC breakdown in the COCs during in vitro maturation.-
dc.description.statementofresponsibilityMaxime Sasseville, Marie-Claude Gagnon, Christine Guillemette, Robert Sullivan, Robert B. Gilchrist and François J. Richard-
dc.publisherEndocrine Soc-
dc.subjectGranulosa Cells-
dc.subjectCells, Cultured-
dc.subjectGap Junctions-
dc.subjectMembrane Microdomains-
dc.subjectMembrane Proteins-
dc.subjectConnexin 43-
dc.subjectLamin Type A-
dc.subjectMicroscopy, Confocal-
dc.subjectBlotting, Western-
dc.subjectGene Expression Regulation, Developmental-
dc.titleRegulation of Gap Junctions in Porcine Cumulus-Oocyte Complexes: Contributions of Granulosa Cell Contact, Gonadotropins, and Lipid Rafts-
dc.typeJournal article-
dc.identifier.orcidGilchrist, R. [0000-0003-1611-7142]-
Appears in Collections:Aurora harvest
Paediatrics publications

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