Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Lactoferrin and desferrioxamine are ineffective in the treatment of Helicobacter pylori infection and may enhance H-pylori growth and gastric inflammation in mice
Author: Huynh, H.
Campbell, M.
Couper, R.
Tran, C.
Lawrence, A.
Butler, R.
Citation: Letters in Applied Microbiology, 2009; 48(5):517-522
Publisher: Blackwell Science Ltd
Issue Date: 2009
ISSN: 0266-8254
Statement of
H.Q. Huynh, M.A.F. Campbell, R.T.L. Couper, C.D. Tran, A. Lawrence and R.N. Butler
Abstract: Aims: To evaluate the efficacy of bovine lactoferrin (BLf), recombinant human lactoferrin (rHLf) and desferrioxamine against Helicobacter pylori in vitro and in mice and also to determine whether BLf or rHLf alter gastric inflammation. Methods and Results: In vitro: Broth dilution susceptibility tests were performed using different concentrations of desferrioxamine, BLf and rHLf. Murine trials: In the prevention trial, C57BL/6 female mice were treated with BLf or rHLF, and then infected with the SS1 strain of H. pylori. In the treatment trial, mice were gavaged with either BLf, rHLf or desferrioxamine. In addition, gastric myeloperoxidase activity (MPO) was measured to assess gastric inflammation. Desferoxamine was found to have a direct bactericidal effect, while BLf and rHLf only partially suppressed H. pylori growth in vitro. However, in both prevention and treatment trials all three forms of treatment failed to reduce H. pylori load in mice. Gastric MPO activity and H. pylori load were noted to be higher with lactoferrin treatments. Conclusions: Our study does not support the use of BLf or rHLF in the treatment of human H. pylori infection. Interestingly, H. pylori growth and gastric inflammation appear to be enhanced by lactoferrin treatment.
Keywords: desferrioxamine; Helicobacter pylori infection; iron chelating agent; lactoferrin; mice; MPO
Description: Journal compilation © 2009 The Society for Applied Microbiology
RMID: 0020090491
DOI: 10.1111/j.1472-765X.2009.02557.x
Appears in Collections:Paediatrics publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.