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Type: Journal article
Title: Metabolic and Mitochondrial Dysfunction in Early Mouse Embryos Following Maternal Dietary Protein Intervention
Author: Mitchell, M.
Schulz, S.
Armstrong, D.
Lane, M.
Citation: Biology of Reproduction, 2009; 80(4):622-630
Publisher: Soc Study Reproduction
Issue Date: 2009
ISSN: 0006-3363
Statement of
Megan Mitchell, Samantha L. Schulz, David T. Armstrong and Michelle Lane
Abstract: Dietary supply of nutrients, both periconception and during pregnancy, influence the growth and development of the fetus and offspring and their health into adult life. Despite the importance of research efforts surrounding the developmental origins of health and disease hypothesis, the biological mechanisms involved remain elusive. Mitochondria are of major importance in the oocyte and early embryo, particularly as a source of ATP generation, and perturbations in their function have been related to reduced embryo quality. The present study examined embryo development following periconception exposure of females to a high-protein diet (HPD) or a low-protein diet (LPD) relative to a medium-protein diet (MPD; control), and we hypothesized that perturbed mitochondrial metabolism in the mouse embryo may be responsible for the impaired embryo and fetal development reported by others. Although the rate of development to the blastocyst stage did not differ between diets, both the HPD and LPD reduced the number of inner cell mass cells in the blastocyst-stage embryo. Furthermore, mitochondrial membrane potential was reduced and mitochondrial calcium levels increased in the 2-cell embryo. Embryos from HPD females had elevated levels of reactive oxygen species and ADP concentrations, indicative of metabolic stress and, potentially, the uncoupling of oxidative phosphorylation, whereas embryos from LPD females had reduced mitochondrial clustering around the nucleus, suggestive of an overall quietening of metabolism. Thus, although periconception dietary supply of different levels of protein is permissive of development, mitochondrial metabolism is altered in the early embryo, and the nature of the perturbation differs between HPD and LPD exposure.
Keywords: Mitochondria
Cleavage Stage, Ovum
Reactive Oxygen Species
Pyruvic Acid
Dietary Proteins
Adenosine Diphosphate
Adenosine Triphosphate
Calcium Signaling
Embryonic Development
Maternal-Fetal Exchange
Models, Biological
Embryo, Mammalian
Maternal Nutritional Physiological Phenomena
Description: Copyright © 2009 by the Biology of Reproduction
DOI: 10.1095/biolreprod.108.072595
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Appears in Collections:Aurora harvest
Obstetrics and Gynaecology publications

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