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Type: Journal article
Title: T cell receptor-mediated signaling induces GRP78 expression in T cells: The implications in maintaining T cell viability
Author: Takano, S.
Ando, T.
Hiramatsu, N.
Kanayama, A.
Maekawa, S.
Ohnuma, Y.
Enomoto, N.
Ogawa, H.
Paton, A.
Paton, J.
Kitamura, M.
Nakao, A.
Citation: Biochemical and Biophysical Research Communications, 2008; 371(4):762-766
Publisher: Academic Press Inc
Issue Date: 2008
ISSN: 0006-291X
Statement of
Shinichi Takano, Takashi Ando, Nobuhiko Hiramatsu, Asuka Kanayama, Shinya Maekawa, Yuko Ohnuma, Nobuyuki Enomoto, Hideoki Ogawa, Adrienne W. Paton, James C. Paton, Masanori Kitamura and Atsuhito Nakao
Abstract: The 78-kDa glucose-regulated protein (GRP78) is an important molecular chaperone in the endoplasmic reticulum (ER) induced by various stresses. This study showed that stimulation with anti-CD3 mAb, PMA plus ionomycin, or an antigen increased the levels of GRP78 mRNA in primary T cells, which was inhibited by Ca2+ chelators EGTA and BAPTA-AM and by an inhibitor of calcineurin FK506. In addition, the specific knockdown of GRP78 protein expression induced apoptosis in mouse EL-4 T cell line associated with CHOP induction and caspase-3 activation. Furthermore, overexpression of GRP78 inhibited PMA/ionomycin-induced cell death in EL-4 cells. Collectively, GRP78 expression is induced by TCR activation via a Ca2+-dependent pathway and may play a critical role in maintaining T cell viability in the steady and TCR-activated states. These results suggest a novel regulatory mechanism and an essential function of GRP78 in T cells.
Keywords: GRP78/BiP
T cells
T cell receptor
Description: Copyright © 2008 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V.
DOI: 10.1016/j.bbrc.2008.04.132
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Appears in Collections:Aurora harvest 5
Molecular and Biomedical Science publications

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