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Type: Journal article
Title: FAT/CD36 expression alone is insufficient to enhance cellular uptake of oleate
Author: Eyre, N.
Cleland, L.
Mayrhofer, G.
Citation: Biochemical and Biophysical Research Communications, 2008; 370(3):404-409
Publisher: Academic Press Inc
Issue Date: 2008
ISSN: 0006-291X
Statement of
Nicholas S. Eyrea, Leslie G. Clelandb and Graham Mayrhofer
Abstract: Fatty acid translocase (FAT/CD36) is one of several proteins implicated in receptor-mediated uptake of long-chain fatty acids (LCFAs). We have tested whether levels of FAT/CD36 correlate with cellular oleic acid import, using a Tet-Off inducible transfected CHO cell line. Consistent with our previous findings, FAT/CD36 was enriched in lipid raft-derived detergent-resistant membranes (DRMs) that also contained caveolin-1, the marker protein of caveolae. Furthermore in transfected cells, plasma membrane FAT/CD36 co-localized extensively with the lipid raft-enriched ganglioside GM1, and partially with a caveolin-1-EGFP fusion protein. Nevertheless, even at high levels of expression, FAT/CD36 did not affect uptake of oleic acid. We propose that the ability of FAT/CD36 to mediate enhanced uptake of LCFAs is dependent on co-expression of other proteins or factors that are lacking in CHO cells.
Keywords: FAT/CD36
Long-chain fatty acid transport
Lipid rafts
Description: Copyright © 2008 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V.
DOI: 10.1016/j.bbrc.2008.02.164
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Appears in Collections:Aurora harvest 5
Molecular and Biomedical Science publications

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