Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/50774
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dc.contributor.authorEyre, N.-
dc.contributor.authorCleland, L.-
dc.contributor.authorMayrhofer, G.-
dc.date.issued2008-
dc.identifier.citationBiochemical and Biophysical Research Communications, 2008; 370(3):404-409-
dc.identifier.issn0006-291X-
dc.identifier.issn1090-2104-
dc.identifier.urihttp://hdl.handle.net/2440/50774-
dc.descriptionCopyright © 2008 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V.-
dc.description.abstractFatty acid translocase (FAT/CD36) is one of several proteins implicated in receptor-mediated uptake of long-chain fatty acids (LCFAs). We have tested whether levels of FAT/CD36 correlate with cellular oleic acid import, using a Tet-Off inducible transfected CHO cell line. Consistent with our previous findings, FAT/CD36 was enriched in lipid raft-derived detergent-resistant membranes (DRMs) that also contained caveolin-1, the marker protein of caveolae. Furthermore in transfected cells, plasma membrane FAT/CD36 co-localized extensively with the lipid raft-enriched ganglioside GM1, and partially with a caveolin-1-EGFP fusion protein. Nevertheless, even at high levels of expression, FAT/CD36 did not affect uptake of oleic acid. We propose that the ability of FAT/CD36 to mediate enhanced uptake of LCFAs is dependent on co-expression of other proteins or factors that are lacking in CHO cells.-
dc.description.statementofresponsibilityNicholas S. Eyrea, Leslie G. Clelandb and Graham Mayrhofer-
dc.language.isoen-
dc.publisherAcademic Press Inc-
dc.source.urihttp://dx.doi.org/10.1016/j.bbrc.2008.02.164-
dc.subjectFAT/CD36-
dc.subjectLong-chain fatty acid transport-
dc.subjectLipid rafts-
dc.subjectCaveolae-
dc.subjectTet-off-
dc.titleFAT/CD36 expression alone is insufficient to enhance cellular uptake of oleate-
dc.typeJournal article-
dc.identifier.doi10.1016/j.bbrc.2008.02.164-
pubs.publication-statusPublished-
dc.identifier.orcidEyre, N. [0000-0002-5424-7573]-
Appears in Collections:Aurora harvest 5
Molecular and Biomedical Science publications

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