Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/50778
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Immunization with Recombinant Murine Cytomegalovirus Expressing Murine Zona Pellucida 3 Causes Permanent Infertility in BALB/c Mice Due to Follicle Depletion and Ovulation Failure
Author: O'Leary, S.
Lloyd, M.
Shellam, G.
Robertson, S.
Citation: Biology of Reproduction, 2008; 79(5):849-860
Publisher: Soc Study Reproduction
Issue Date: 2008
ISSN: 0006-3363
1529-7268
Statement of
Responsibility: 
Sean O'Leary, Megan L. Lloyd, Geoffrey R. Shellam and Sarah A. Robertson
Abstract: Zona pellucida (ZP) glycoproteins are promising candidate antigens for use in immunocontraceptive vaccines because of their crucial role in mammalian fertilization. A single intraperitoneal immunization with recombinant murine cytomegalovirus engineered to express murine ZP3 (rMCMV-mZP3) induces permanent infertility with no evident systemic illness in female BALB/c mice. To investigate the mechanisms underpinning reproductive failure elicited by rMCMV-mZP3, ovarian parameters and reproductive function were evaluated at time points spanning 10 days to 5 wk after virus inoculation. Fertility was substantially impaired by 14 days after inoculation with rMCMV-mZP3 and was fully ablated by 21 days. Pregnancies established after inoculation but before complete infertility showed no adverse effects on fetal viability assessed at Day 17.5 post coitum (pc). Infertile mice retained estrous cycling activity and remained receptive to mating; however, at Day 3.5 pc there were fewer developing embryos and corpora lutea, plasma progesterone content was reduced, and there was no evidence of excess unfertilized oocytes. Consistent with this, profound ovarian pathology was evident from 10 days after rMCMV-mZP3 inoculation, with a decline first in mature ovarian follicles and then in immature ovarian follicles and with diminished expression of genes regulating follicle development, including Nobox, Gdf9, and Gja1 (connexin43). Follicle loss was associated with mild focal oophoritis and with recruitment of inflammatory leukocytes, predominantly CD4+ and CD8+ T cells evident from 10 days after virus inoculation. These data indicate that vaccination with rMCMV-mZP3 causes permanent infertility in BALB/c mice principally due to induction of ovarian autoimmune pathology leading to progressive oocyte depletion and eventual ovulation failure..
Keywords: Ovary; Ovarian Follicle; Leukocytes; Animals; Mice, Inbred BALB C; Mice; Cytomegalovirus; Infertility, Female; Homeodomain Proteins; Egg Proteins; Membrane Glycoproteins; Connexin 43; Receptors, Cell Surface; Vaccines, Synthetic; Transcription Factors; Vaccines, Contraceptive; Vaccination; Embryonic Development; Fetal Development; Ovulation; Pregnancy; Time Factors; Female; Male; Growth Differentiation Factor 9; Zona Pellucida Glycoproteins
Description: Copyright © 2009 by the Biology of Reproduction
RMID: 0020083355
DOI: 10.1095/biolreprod.108.067884
Appears in Collections:Obstetrics and Gynaecology publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.