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Type: Journal article
Title: Androgen receptor inhibits estrogen receptor-alpha activity and is prognostic in breast cancer
Author: Peters, A.
Buchanan, G.
Ricciardelli, C.
Bianco-Miotto, T.
Centenera, M.
Harris, J.
Jindal, S.
Segara, D.
Jia, L.
Moore, N.
Henshall, S.
Birrell, S.
Coetzee, G.
Sutherland, R.
Butler, L.
Tilley, W.
Citation: Cancer Research, 2009; 69(15):6131-6140
Publisher: Amer Assoc Cancer Research
Issue Date: 2009
ISSN: 0008-5472
Statement of
Amelia A. Peters, Grant Buchanan, Carmela Ricciardelli, Tina Bianco-Miotto, Margaret M. Centenera, Jonathan M. Harris, Shalini Jindal, Davendra Segara, Li Jia, Nicole L. Moore, Susan M. Henshall, Stephen N. Birrell, Gerhard A. Coetzee, Robert L. Sutherland, Lisa M. Butler and Wayne D. Tilley
Abstract: There is emerging evidence that the balance between estrogen receptor-alpha (ER(alpha)) and androgen receptor (AR) signaling is a critical determinant of growth in the normal and malignant breast. In this study, we assessed AR status in a cohort of 215 invasive ductal breast carcinomas. AR and (ER(alpha)) were coexpressed in the majority (80-90%) of breast tumor cells. Kaplan-Meier product limit analysis and multivariate Cox regression showed that AR is an independent prognostic factor in (ER(alpha))-positive disease, with a low level of AR (less than median of 75% positive cells) conferring a 4.6-fold increased risk of cancer-related death (P = 0.002). Consistent with a role for AR in breast cancer outcome, AR potently inhibited (ER(alpha))transactivation activity and 17beta-estradiol-stimulated growth of breast cancer cells. Transfection of MDA-MB-231 breast cancer cells with either functionally impaired AR variants or the DNA-binding domain of the AR indicated that the latter is both necessary and sufficient for inhibition of (ER(alpha)) signaling. Consistent with molecular modeling, electrophoretic mobility shift assays showed binding of the AR to an estrogen-responsive element (ERE). Evidence for a functional interaction of the AR with an ERE in vivo was provided by chromatin immunoprecipitation data, revealing recruitment of the AR to the progesterone receptor promoter in T-47D breast cancer cells. We conclude that, by binding to a subset of EREs, the AR can prevent activation of target genes that mediate the stimulatory effects of 17beta-estradiol on breast cancer cells.
Keywords: COS Cells
Cell Line, Tumor
Carcinoma, Ductal, Breast
Breast Neoplasms
Receptors, Androgen
Estrogen Receptor alpha
DNA, Neoplasm
Signal Transduction
Response Elements
Models, Molecular
Transcriptional Activation
Chlorocebus aethiops
Description: © 2009 American Association for Cancer Research
DOI: 10.1158/0008-5472.CAN-09-0452
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