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https://hdl.handle.net/2440/51175
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Type: | Journal article |
Title: | Localizing the chemical forms of sulfur in vivo using X-ray fluorescence spectroscopic imaging: application to onion (Allium cepa) tissues |
Author: | Pickering, I. Sneeden, E. Prince, R. Block, E. Harris, H. Hirsch, G. George, G. |
Citation: | Biochemistry, 2009; 48(29):6846-6853 |
Publisher: | Amer Chemical Soc |
Issue Date: | 2009 |
ISSN: | 0006-2960 1520-4995 |
Statement of Responsibility: | Ingrid J. Pickering, Eileen Yu Sneeden, Roger C. Prince, Eric Block, Hugh H. Harris, Gregory Hirsch and Graham N. George |
Abstract: | Sulfur has a particularly rich biochemistry and fills a number of important roles in biology. In situ information on sulfur biochemistry is generally difficult to obtain because of a lack of biophysical techniques that have sufficient sensitivity to molecular form. We have recently reported that sulfur K-edge X-ray absorption spectroscopy can be used as a direct probe of the sulfur biochemistry of living mammalian cells [Gnida, M., et al. (2007) Biochemistry 46, 14735-14741]. Here we report an extension of this work and develop sulfur K-edge X-ray fluorescence spectroscopic imaging as an in vivo probe of sulfur metabolism in living cells. For this work, we have chosen onion (Allium cepa) as a tractable model system with well-developed sulfur biochemistry and present evidence of the localization of a number of different chemical forms. X-ray absorption spectroscopy of onion sections showed increased levels of lachrymatory factor (LF) and thiosulfinate and decreased levels of sulfoxide (LF precursor) following cell breakage. In intact cells, X-ray fluorescence spectroscopic imaging showed elevated levels of sulfoxides in the cytosol and elevated levels of reduced sulfur in the central transport vessels and bundle sheath cells. |
Keywords: | Onions Sulfur Molecular Probes Spectrometry, Fluorescence X-Rays |
Description: | Copyright © 2009 American Chemical Society |
DOI: | 10.1021/bi900368x |
Published version: | http://dx.doi.org/10.1021/bi900368x |
Appears in Collections: | Aurora harvest Chemistry and Physics publications Environment Institute publications |
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