Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/51175
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dc.contributor.authorPickering, I.-
dc.contributor.authorSneeden, E.-
dc.contributor.authorPrince, R.-
dc.contributor.authorBlock, E.-
dc.contributor.authorHarris, H.-
dc.contributor.authorHirsch, G.-
dc.contributor.authorGeorge, G.-
dc.date.issued2009-
dc.identifier.citationBiochemistry, 2009; 48(29):6846-6853-
dc.identifier.issn0006-2960-
dc.identifier.issn1520-4995-
dc.identifier.urihttp://hdl.handle.net/2440/51175-
dc.descriptionCopyright © 2009 American Chemical Society-
dc.description.abstractSulfur has a particularly rich biochemistry and fills a number of important roles in biology. In situ information on sulfur biochemistry is generally difficult to obtain because of a lack of biophysical techniques that have sufficient sensitivity to molecular form. We have recently reported that sulfur K-edge X-ray absorption spectroscopy can be used as a direct probe of the sulfur biochemistry of living mammalian cells [Gnida, M., et al. (2007) Biochemistry 46, 14735-14741]. Here we report an extension of this work and develop sulfur K-edge X-ray fluorescence spectroscopic imaging as an in vivo probe of sulfur metabolism in living cells. For this work, we have chosen onion (Allium cepa) as a tractable model system with well-developed sulfur biochemistry and present evidence of the localization of a number of different chemical forms. X-ray absorption spectroscopy of onion sections showed increased levels of lachrymatory factor (LF) and thiosulfinate and decreased levels of sulfoxide (LF precursor) following cell breakage. In intact cells, X-ray fluorescence spectroscopic imaging showed elevated levels of sulfoxides in the cytosol and elevated levels of reduced sulfur in the central transport vessels and bundle sheath cells.-
dc.description.statementofresponsibilityIngrid J. Pickering, Eileen Yu Sneeden, Roger C. Prince, Eric Block, Hugh H. Harris, Gregory Hirsch and Graham N. George-
dc.language.isoen-
dc.publisherAmer Chemical Soc-
dc.source.urihttp://dx.doi.org/10.1021/bi900368x-
dc.subjectOnions-
dc.subjectSulfur-
dc.subjectMolecular Probes-
dc.subjectSpectrometry, Fluorescence-
dc.subjectX-Rays-
dc.titleLocalizing the chemical forms of sulfur in vivo using X-ray fluorescence spectroscopic imaging: application to onion (Allium cepa) tissues-
dc.typeJournal article-
dc.identifier.doi10.1021/bi900368x-
pubs.publication-statusPublished-
dc.identifier.orcidHarris, H. [0000-0002-3472-8628]-
Appears in Collections:Aurora harvest
Chemistry and Physics publications
Environment Institute publications

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