Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/51175
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pickering, I. | - |
dc.contributor.author | Sneeden, E. | - |
dc.contributor.author | Prince, R. | - |
dc.contributor.author | Block, E. | - |
dc.contributor.author | Harris, H. | - |
dc.contributor.author | Hirsch, G. | - |
dc.contributor.author | George, G. | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Biochemistry, 2009; 48(29):6846-6853 | - |
dc.identifier.issn | 0006-2960 | - |
dc.identifier.issn | 1520-4995 | - |
dc.identifier.uri | http://hdl.handle.net/2440/51175 | - |
dc.description | Copyright © 2009 American Chemical Society | - |
dc.description.abstract | Sulfur has a particularly rich biochemistry and fills a number of important roles in biology. In situ information on sulfur biochemistry is generally difficult to obtain because of a lack of biophysical techniques that have sufficient sensitivity to molecular form. We have recently reported that sulfur K-edge X-ray absorption spectroscopy can be used as a direct probe of the sulfur biochemistry of living mammalian cells [Gnida, M., et al. (2007) Biochemistry 46, 14735-14741]. Here we report an extension of this work and develop sulfur K-edge X-ray fluorescence spectroscopic imaging as an in vivo probe of sulfur metabolism in living cells. For this work, we have chosen onion (Allium cepa) as a tractable model system with well-developed sulfur biochemistry and present evidence of the localization of a number of different chemical forms. X-ray absorption spectroscopy of onion sections showed increased levels of lachrymatory factor (LF) and thiosulfinate and decreased levels of sulfoxide (LF precursor) following cell breakage. In intact cells, X-ray fluorescence spectroscopic imaging showed elevated levels of sulfoxides in the cytosol and elevated levels of reduced sulfur in the central transport vessels and bundle sheath cells. | - |
dc.description.statementofresponsibility | Ingrid J. Pickering, Eileen Yu Sneeden, Roger C. Prince, Eric Block, Hugh H. Harris, Gregory Hirsch and Graham N. George | - |
dc.language.iso | en | - |
dc.publisher | Amer Chemical Soc | - |
dc.source.uri | http://dx.doi.org/10.1021/bi900368x | - |
dc.subject | Onions | - |
dc.subject | Sulfur | - |
dc.subject | Molecular Probes | - |
dc.subject | Spectrometry, Fluorescence | - |
dc.subject | X-Rays | - |
dc.title | Localizing the chemical forms of sulfur in vivo using X-ray fluorescence spectroscopic imaging: application to onion (Allium cepa) tissues | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1021/bi900368x | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Harris, H. [0000-0002-3472-8628] | - |
Appears in Collections: | Aurora harvest Chemistry and Physics publications Environment Institute publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.