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|Title:||Local gene transfer to modulate rat corneal allograft rejection|
|Citation:||Investigative Ophthalmology & Visual Science, 2005; 46(5):1675-1681|
|Publisher:||Assoc Research Vision Ophthalmology Inc|
|Claire F. Jessup, Helen M. Brereton, Pamela J. Sykes, Michael A. Thiel, Douglas J. Coster and Keryn A. Williams|
|Abstract:||PURPOSE. Allograft rejection is the leading cause of corneal graft failure. CD4+ T cells control the allograft response and represent targets for antirejection therapy. The purpose of this study was to transfer cDNA encoding a monomeric anti-CD4 antibody fragment to donor corneal endothelium, to attempt to modulate orthotopic corneal allograft rejection in the rat. METHODS. A replication-deficient adenoviral vector (AdV) encoding anti-CD4 single-chain, variable-domain antibody fragment (scFv) and enhanced green fluorescent protein (eGFP) was constructed (AdCD4GFP). AdV encoding eGFP alone (AdGFP) was used as a control. Transgenic product was detected by reverse transcription–polymerase chain reaction (RT-PCR), Western blot, flow cytometry, and fluorescence microscopy. The alloinhibitory capacity of anti-rat CD4 scFv was measured in the one-way mixed lymphocyte reaction (MLR). The survival of Wistar-Furth corneas transduced with AdV either immediately or 3 days before orthotopic transplantation in Fischer 344 recipients was examined. RESULTS. ScFv and eGFP mRNAs were detected in rat corneas transduced in vitro, and active scFv secreted in corneal supernatants peaked at days 4 to 5 after transduction at 23 ± 4 ng of protein per cornea per day. Antibody and scFv against rat CD4 blocked alloproliferation in MLR. However, transduction of corneas with AdCD4GFP ex vivo, immediately before transplantation, or in vivo, 3 days before transplantation, did not significantly prolong corneal allograft survival (P > 0.05). CONCLUSIONS. Anti-CD4 scFvs were capable of blocking allostimulation, but their local expression within the eye did not prolong corneal allograft survival, suggesting that sensitization may still occur.|
|Keywords:||Cornea; CD4-Positive T-Lymphocytes; Animals; Rats, Inbred F344; Rats, Inbred WF; Rats; Adenoviridae; Immunoglobulin Variable Region; Luminescent Proteins; Green Fluorescent Proteins; Recombinant Fusion Proteins; RNA, Messenger; Microscopy, Fluorescence; Lymphocyte Culture Test, Mixed; Blotting, Western; Corneal Transplantation; Transplantation, Homologous; Flow Cytometry; Gene Transfer Techniques; Cell Proliferation; Graft Rejection; Graft Survival; Genetic Vectors; Single-Chain Antibodies|
|Description:||Copyright © 2005 by the Association for Research in Vision and Ophthalmology|
|Appears in Collections:||Medicine publications|
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