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Type: Journal article
Title: Local gene transfer to modulate rat corneal allograft rejection
Author: Jessup, C.
Brereton, H.
Sykes, P.
Thiel, M.
Coster, D.
Williams, K.
Citation: Investigative Ophthalmology and Visual Science, 2005; 46(5):1675-1681
Publisher: Assoc Research Vision Ophthalmology Inc
Issue Date: 2005
ISSN: 0146-0404
Statement of
Claire F. Jessup, Helen M. Brereton, Pamela J. Sykes, Michael A. Thiel, Douglas J. Coster and Keryn A. Williams
Abstract: PURPOSE. Allograft rejection is the leading cause of corneal graft failure. CD4+ T cells control the allograft response and represent targets for antirejection therapy. The purpose of this study was to transfer cDNA encoding a monomeric anti-CD4 antibody fragment to donor corneal endothelium, to attempt to modulate orthotopic corneal allograft rejection in the rat. METHODS. A replication-deficient adenoviral vector (AdV) encoding anti-CD4 single-chain, variable-domain antibody fragment (scFv) and enhanced green fluorescent protein (eGFP) was constructed (AdCD4GFP). AdV encoding eGFP alone (AdGFP) was used as a control. Transgenic product was detected by reverse transcription–polymerase chain reaction (RT-PCR), Western blot, flow cytometry, and fluorescence microscopy. The alloinhibitory capacity of anti-rat CD4 scFv was measured in the one-way mixed lymphocyte reaction (MLR). The survival of Wistar-Furth corneas transduced with AdV either immediately or 3 days before orthotopic transplantation in Fischer 344 recipients was examined. RESULTS. ScFv and eGFP mRNAs were detected in rat corneas transduced in vitro, and active scFv secreted in corneal supernatants peaked at days 4 to 5 after transduction at 23 ± 4 ng of protein per cornea per day. Antibody and scFv against rat CD4 blocked alloproliferation in MLR. However, transduction of corneas with AdCD4GFP ex vivo, immediately before transplantation, or in vivo, 3 days before transplantation, did not significantly prolong corneal allograft survival (P > 0.05). CONCLUSIONS. Anti-CD4 scFvs were capable of blocking allostimulation, but their local expression within the eye did not prolong corneal allograft survival, suggesting that sensitization may still occur.
Keywords: Cornea
CD4-Positive T-Lymphocytes
Rats, Inbred F344
Rats, Inbred WF
Immunoglobulin Variable Region
Luminescent Proteins
Green Fluorescent Proteins
Recombinant Fusion Proteins
RNA, Messenger
Microscopy, Fluorescence
Lymphocyte Culture Test, Mixed
Blotting, Western
Corneal Transplantation
Transplantation, Homologous
Flow Cytometry
Gene Transfer Techniques
Cell Proliferation
Graft Rejection
Graft Survival
Genetic Vectors
Single-Chain Antibodies
Description: Copyright © 2005 by the Association for Research in Vision and Ophthalmology
DOI: 10.1167/iovs.04-1140
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