Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Attenuation of Flightless I, an actin-remodelling protein, improves burn injury repair via modulation of transforming growth factor (TGF)-beta 1 and TGF-beta 3|
|Citation:||British Journal of Dermatology, 2009; 161(2):326-336|
|Publisher:||Blackwell Science Ltd|
|D.H. Adams, N. Ruzehaji, X.L. Strudwick, J.E. Greenwood, H.D. Campbell, R. Arkell and A.J. Cowin|
|Abstract:||<h4>Background</h4>The pathophysiological mechanisms involved in burn injury repair are still not fully understood but include processes involving cellular proliferation, migration and adhesion. The actin cytoskeleton is intricately involved in these key wound repair processes. Flightless I (Flii), an actin-remodelling protein and transcriptional regulator, is an important regulator of wound healing.<h4>Objectives</h4>To investigate the function of Flii gene expression in burn injury repair.<h4>Methods</h4>Partial-thickness scald wounds were created on Flii heterozygous (Flii(+/-)), wild-type (WT) and Flii transgenic (Flii(Tg/+)) mice. Burns were assessed using histology and immunohistochemistry, real-time quantitative polymerase chain reaction and biochemical analysis.<h4>Results</h4>Flii expression, while upregulated in burn injuries, was significantly lower in the wounds of Flii(+/-) vs. WT vs. Flii(Tg/+) mice and healing was improved in Flii(+/-) mice with their burns healing faster than WT and Flii(Tg/+). Pro-scarring transforming growth factor (TGF)-beta1 protein and gene expression were reduced in Flii(+/-) burns while antiscarring TGF-beta3 was significantly elevated. Anti-alpha-smooth muscle actin (alpha-SMA) was decreased in Flii(+/-) burns suggesting a decrease in contractile myofibroblasts in the developing scars. Although Flii is primarily a nuclear and cytoplasmic protein it is also released by wounded cells. Intradermal injection of Flii-neutralizing antibodies (FliAbs) to WT burn wounds significantly improved their healing, indicating a potential novel approach for treating burns. Decreased TGF-beta1 and elevated TGF-beta3 expression were observed in FliAb-treated burns, which may contribute to their observed improvement in healing.<h4>Conclusions</h4>Strategies aimed at reducing Flii expression, for example using neutralizing antibodies, may lead to improved burn outcomes.|
Mice, Inbred BALB C
Transforming Growth Factor beta1
Transforming Growth Factor beta3
|Description:||The definitive version may be found at www.wiley.com|
|Appears in Collections:||Aurora harvest 5|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.