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Type: Journal article
Title: Alcohol metabolism increases the replication of hepatitis C virus and attenuates the antiviral action of interferon
Author: McCartney, E.
Semendric, L.
Helbig, K.
Hinze, S.
Jones, B.
Weinman, S.
Beard, M.
Citation: Journal of Infectious Diseases, 2008; 198(12):1766-1775
Publisher: Univ Chicago Press
Issue Date: 2008
ISSN: 0022-1899
Statement of
Erin M. McCartney, Ljiljana Semendric, Karla J. Helbig, Susan Hinze, Brett Jones, Steven A. Weinman and Michael R. Beard
Abstract: The interactions between hepatitis C virus (HCV) and alcohol metabolism are not well understood. To determine the effect that alcohol metabolism has on HCV replication and the antiviral action of interferon (IFN), Huh-7 cells that harbor HCV replication and metabolize ethanol via the introduced expression of cytochrome P450 2E1 (Cyp2e1) were treated with ethanol and IFN-alpha. Treatment of these cells with ethanol (0-100 mmol/L) significantly increased HCV replication. This effect was dependent on Cyp2e1 expression and alcohol-metabolized oxidative stress (OS), because the antioxidant N-acetylcysteine blocked this effect. Furthermore, the anti-HCV action of IFN-alpha was attenuated in the presence of ethanol metabolism, most likely via attenuation of Stat1 tyrosine-701 phosphorylation. These in vitro results mimic what is often noted clinically, and further dissection of this model system will aid in our understanding of interactions between HCV and alcohol metabolism.
Keywords: Cell Line
Cytochrome P-450 CYP2E1
Virus Replication
Gene Expression Regulation, Enzymologic
Oxidative Stress
DOI: 10.1086/593216
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Appears in Collections:Aurora harvest 5
Molecular and Biomedical Science publications

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