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dc.contributor.authorPalmer, A.en
dc.contributor.authorAhlgren-Berg, A.en
dc.contributor.authorEgan, J.en
dc.contributor.authorDodd, I.en
dc.contributor.authorShearwin, K.en
dc.identifier.citationMolecular Cell, 2009; 34(5):545-555en
dc.description.abstractElongating RNA polymerases (RNAPs) can interfere with transcription from downstream promoters by inhibiting DNA binding by RNAP and activators. However, combining quantitative measurement with mathematical modeling, we show that simple RNAP elongation cannot produce the strong asymmetric interference observed between a natural face-to-face promoter pair in bacteriophage lambda. Pausing of elongating polymerases over the RNAP-binding site of the downstream promoter is demonstrated in vivo and is shown by modeling to account for the increased interference. The model successfully predicts the effects on interference of treatments increasing or reducing pausing. Gene regulation by pausing-enhanced occlusion provides a general and potentially widespread mechanism by which even weak converging or tandem transcription, either coding or noncoding, can bring about strong in cis repression.en
dc.description.statementofresponsibilityAdam C. Palmer, Alexandra Ahlgren-Berg, J. Barry Egan, Ian B. Dodd and Keith E. Shearwinen
dc.publisherCell Pressen
dc.rights© 2009 Elsevier Inc.en
dc.subjectEscherichia coli; Bacteriophage lambda; DNA-Directed RNA Polymerases; Transcription, Genetic; Gene Expression Regulation; Binding Sites; Mutation; Models, Genetic; Promoter Regions, Geneticen
dc.titlePotent transcriptional interference by pausing of RNA polymerases over a downstream promoteren
dc.typeJournal articleen
pubs.library.collectionMolecular and Biomedical Science publicationsen
Appears in Collections:Molecular and Biomedical Science publications

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