Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/51757
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Type: Journal article
Title: Effect of solution environment on the purification of pertussis toxin
Author: Wu, T.
Huang, Y.
Bi, J.
Zhang, Y.
Sun, L.
Sun, C.
Su, Z.
Citation: Chinese Journal of Biotechnology, 2008; 24(7):1279-1284
Publisher: Allerton Press, Inc.
Issue Date: 2008
ISSN: 1042-749X
1872-2075
Statement of
Responsibility: 
Tie Wu, Yongdong Huang, Jingxiu Bi, Yan Zhang, Lijing Sun, Chunbao Sun and Zhiguo Su
Abstract: The low recovery of pertussis toxin (PT) and the low resolving efficiency of chromatography, due to the instability of PT in low salt condition, are the main challenges for its purification. We aplied 2 mol/L urea to prevent the aggregation and disassociation of PT during the purification by ion-exchange chromatography (IEC) and gel filtration chromatography (GFC). The effect of urea on the purification of PT was studied by ELISA assay and non-reduced SDS-PAGE. The activity recoveries of PT and filamentous hemagglutinin (FHA) in IEC and GFC, the resolution efficiency in GFC and the purities of PT and FHA were improved obviously by adding 2 mol/L urea in the mobile phase. The results highlight the potential application of urea in the acellular pertussis vaccine (APV) manufacture procedure.
Keywords: Humans; Urea; Pertussis Toxin; Adhesins, Bacterial; Virulence Factors, Bordetella; Pertussis Vaccine; Vaccines, Acellular; Solutions; Chromatography, Ion Exchange
RMID: 0020083466
DOI: 10.1016/S1872-2075(08)60061-8
Appears in Collections:Chemical Engineering publications

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