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Type: Journal article
Title: Methylation of TIMP3 in esophageal squamous cell carcinoma
Author: Smith, E.
De Young, N.
Tian, Z.
Caruso, M.
Ruszkiewicz, A.
Liu, J.
Jamieson, G.
Drew, P.
Citation: World Journal of Gastroenterology, 2008; 14(2):203-210
Publisher: W J G Press
Issue Date: 2008
ISSN: 1007-9327
Statement of
Eric Smith, Neville J De Young, Zi-Qiang Tian, Maria Caruso, Andrew R Ruszkiewicz, Jun-Feng Liu, Glyn G Jamieson, Paul A Drew
Abstract: <h4>Aim</h4>To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3) promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China.<h4>Methods</h4>Cancer cell lines were treated with or without the demethylating reagent 5-aza-2'-deoxycytidine. Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR. Tumors and proximal resection margins were obtained from 64 patients with esophageal squamous cell carcinoma from a region of high incidence in China. Methylation was assessed by melt curve analysis and expression by immunohistochemistry.<h4>Results</h4>Methylation in one of the three promoter regions assessed correlated with gene silencing in esophageal cell lines. A degree of methylation of TIMP3 was found in only four esophageal squamous cell carcinomas, and partial loss of TIMP3 protein expression in just one.<h4>Conclusion</h4>Methylation and loss of expression of TIMP3 occurs infrequently in esophageal squamous cell carcinoma in a region of high incidence in China.
Keywords: Cell Line, Tumor
Carcinoma, Squamous Cell
Esophageal Neoplasms
Tissue Inhibitor of Metalloproteinase-3
DNA Methylation
Gene Expression Regulation, Neoplastic
Middle Aged
DOI: 10.3748/wjg.14.203
Appears in Collections:Aurora harvest
Surgery publications

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