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|Title:||Methylation of TIMP3 in esophageal squamous cell carcinoma|
De Young, N.
|Citation:||World Journal of Gastroenterology, 2008; 14(2):203-210|
|Publisher:||W J G Press|
|Eric Smith, Neville J De Young, Zi-Qiang Tian, Maria Caruso, Andrew R Ruszkiewicz, Jun-Feng Liu, Glyn G Jamieson, Paul A Drew|
|Abstract:||<h4>Aim</h4>To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3) promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China.<h4>Methods</h4>Cancer cell lines were treated with or without the demethylating reagent 5-aza-2'-deoxycytidine. Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR. Tumors and proximal resection margins were obtained from 64 patients with esophageal squamous cell carcinoma from a region of high incidence in China. Methylation was assessed by melt curve analysis and expression by immunohistochemistry.<h4>Results</h4>Methylation in one of the three promoter regions assessed correlated with gene silencing in esophageal cell lines. A degree of methylation of TIMP3 was found in only four esophageal squamous cell carcinomas, and partial loss of TIMP3 protein expression in just one.<h4>Conclusion</h4>Methylation and loss of expression of TIMP3 occurs infrequently in esophageal squamous cell carcinoma in a region of high incidence in China.|
|Keywords:||Cell Line, Tumor|
Carcinoma, Squamous Cell
Tissue Inhibitor of Metalloproteinase-3
Gene Expression Regulation, Neoplastic
|Appears in Collections:||Aurora harvest|
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