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dc.contributor.authorSmith, E.-
dc.contributor.authorDe Young, N.-
dc.contributor.authorTian, Z.-
dc.contributor.authorCaruso, M.-
dc.contributor.authorRuszkiewicz, A.-
dc.contributor.authorLiu, J.-
dc.contributor.authorJamieson, G.-
dc.contributor.authorDrew, P.-
dc.identifier.citationWorld Journal of Gastroenterology, 2008; 14(2):203-210-
dc.description.abstract<h4>Aim</h4>To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3) promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China.<h4>Methods</h4>Cancer cell lines were treated with or without the demethylating reagent 5-aza-2'-deoxycytidine. Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR. Tumors and proximal resection margins were obtained from 64 patients with esophageal squamous cell carcinoma from a region of high incidence in China. Methylation was assessed by melt curve analysis and expression by immunohistochemistry.<h4>Results</h4>Methylation in one of the three promoter regions assessed correlated with gene silencing in esophageal cell lines. A degree of methylation of TIMP3 was found in only four esophageal squamous cell carcinomas, and partial loss of TIMP3 protein expression in just one.<h4>Conclusion</h4>Methylation and loss of expression of TIMP3 occurs infrequently in esophageal squamous cell carcinoma in a region of high incidence in China.-
dc.description.statementofresponsibilityEric Smith, Neville J De Young, Zi-Qiang Tian, Maria Caruso, Andrew R Ruszkiewicz, Jun-Feng Liu, Glyn G Jamieson, Paul A Drew-
dc.publisherW J G Press-
dc.subjectCell Line, Tumor-
dc.subjectCarcinoma, Squamous Cell-
dc.subjectEsophageal Neoplasms-
dc.subjectTissue Inhibitor of Metalloproteinase-3-
dc.subjectDNA Methylation-
dc.subjectGene Expression Regulation, Neoplastic-
dc.subjectMiddle Aged-
dc.titleMethylation of TIMP3 in esophageal squamous cell carcinoma-
dc.typeJournal article-
dc.identifier.orcidSmith, E. [0000-0003-2958-3492]-
dc.identifier.orcidRuszkiewicz, A. [0000-0001-9052-4948]-
dc.identifier.orcidDrew, P. [0000-0001-5661-4771]-
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Surgery publications

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