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Type: Journal article
Title: Circulating RANKL is inversely related to RANKL mRNA levels in bone in osteoarthritic males
Author: Findlay, D.
Chehade, M.
Tsangari, H.
Neale, S.
Hay, S.
Hopwood, B.
Pannach, S.
O'Loughlin, P.
Fazzalari, N.
Citation: Arthritis Research & Therapy, 2008; 10(1):1-9
Publisher: BioMed Central Ltd.
Issue Date: 2008
ISSN: 1478-6362
Statement of
David Findlay, Mellick Chehade, Helen Tsangari, Susan Neale, Shelley Hay, Blair Hopwood, Susan Pannach, Peter O'Loughlin and Nicola Fazzalari
Abstract: Introduction The relationship of circulating levels of receptor activator of nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG) with the expression of these molecules in bone has not been established. The objective of this study was to measure, in humans, the serum levels of RANKL and OPG, and the corresponding levels in bone of mRNA encoding these proteins. Methods Fasting blood samples were obtained on the day of surgery from patients presenting for hip replacement surgery for primary osteoarthritis (OA). Intraoperatively, samples of intertrochanteric trabecular bone were collected for analysis of OPG and RANKL mRNA, using real time RT-PCR. Samples were obtained from 40 patients (15 men with age range 50 to 79 years, and 25 women with age range 47 to 87 years). Serum total RANKL and free OPG levels were measured using ELISA. Results Serum OPG levels increased over the age range of this cohort. In the men RANKL mRNA levels were positively related to age, whereas serum RANKL levels were negatively related to age. Again, in the men serum RANKL levels were inversely related (r = -0.70, P = 0.007) to RANKL mRNA levels. Also in the male group, RANKL mRNA levels were associated with a number of indices of bone structure (bone volume fraction relative to bone tissue volume, specific surface of bone relative to bone tissue volume, and trabecular thickness), bone remodelling (eroded surface and osteoid surface), and biochemical markers of bone turnover (serum alkaline phosphatase and osteocalcin, and urinary deoxypyridinoline). Conclusion This is the first report to show a relationship between serum RANKL and the expression of RANKL mRNA in bone.
Keywords: Bone and Bones; Femur; Humans; Osteoarthritis; RNA, Messenger; Sex Factors; Bone Remodeling; Aged; Aged, 80 and over; Middle Aged; Female; Male; RANK Ligand; Osteoprotegerin
Rights: © 2008 Findlay et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020080514
DOI: 10.1186/ar2348
Appears in Collections:Orthopaedics and Trauma publications

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