Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/52565
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dc.contributor.authorGeary, S.-
dc.contributor.authorCowin, A.-
dc.contributor.authorCopeland, B.-
dc.contributor.authorBaleato, R.-
dc.contributor.authorMiyazaki, K.-
dc.contributor.authorAshman, L.-
dc.date.issued2008-
dc.identifier.citationExperimental Cell Research, 2008; 314(11-12):2165-2175-
dc.identifier.issn0014-4827-
dc.identifier.issn1090-2422-
dc.identifier.urihttp://hdl.handle.net/2440/52565-
dc.description.abstractPrevious studies showed that CD151-null mice have a skin wound healing deficit. To gain an understanding of the role of CD151 in re-epithelialisation and dermal contraction, keratinocyte and fibroblast functions were assayed. Primary CD151-null keratinocytes displayed defective migration on Matrigel (a basement membrane equivalent) and laminin-332, the primary adhesion component of basement membranes, but not on collagen-I. Adhesion, spreading and proliferation were also deficient on laminin-332, but not collagen-I. The data suggest that loss of CD151 impairs the function of its primary interaction partners, integrin alpha3beta1- and/or alpha6beta4 which bind to laminin-332. Skin fibroblasts also produce CD151 mRNA. CD151-null fibroblasts migrated significantly faster on collagen I than wild type fibroblasts, confirming that they possess functional collagen receptors. However, no significant decrease in the ability of CD151-null fibroblasts to cause contraction in floating collagen gel assays in response to transforming growth factor beta-1 (TGF-beta1) or platelet derived growth factor (PDGF-BB) was observed, nor was there an effect on fibroblast adhesion or proliferation on collagen-I. The data implicate CD151 as a facilitator of laminin-332-mediated keratinocyte functions that impact on the re-epithelialisation process intrinsic to wound healing and further suggest a potential novel role for CD151 in fibroblast migration.-
dc.description.statementofresponsibilitySean M. Geary, Allison J. Cowin, Ben Copeland, Rosa M. Baleato, Kaoru Miyazaki, Leonie K. Ashman-
dc.language.isoen-
dc.publisherAcademic Press Inc Elsevier Science-
dc.subjectTetraspanin-
dc.subjectSkin wound healing-
dc.subjectLaminin-332-
dc.subjectRe-epithelialization-
dc.subjectCD151-
dc.subjectKeratinocyte-
dc.subjectFibroblast-
dc.titleThe role of the tetraspanin CD151 in primary keratinocyte and fibroblast functions: Implications for wound healing-
dc.typeJournal article-
dc.identifier.doi10.1016/j.yexcr.2008.04.011-
pubs.publication-statusPublished-
dc.identifier.orcidCowin, A. [0000-0003-2885-2080]-
Appears in Collections:Aurora harvest
Paediatrics publications

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