Please use this identifier to cite or link to this item:
Scopus Web of ScienceĀ® Altmetric
Type: Journal article
Title: Investigating glutamate receptor-like gene co-expression in Arabidopsis thaliana
Author: Roy, S.
Gilliham, M.
Berger, B.
Essah, P.
Cheffings, C.
Miller, A.
Davenport, R.
Liu, L.
Skynner, M.
Davies, J.
Richardson, P.
Leigh, R.
Tester, M.
Citation: Plant, Cell and Environment, 2008; 31(6):861-871
Publisher: Blackwell Publishing Ltd
Issue Date: 2008
ISSN: 0140-7791
Statement of
S. J. Roy, M. Gilliham, B. Berger, P. A. Essah, C. Cheffings, A. J. Miller, R. J. Davenport, L.-H. Liu, M. J. Skynner, J. M. Davies, P. Richardson, R. A. Leigh & M. Tester
Abstract: There is increasing evidence of the important roles of glutamate receptors (GLRs) in plant development and in adaptation to stresses. However, the studies of these putative ion channels, both in planta and in Xenopus oocytes, may have been limited by our lack of knowledge of possible GLR heteromer formation in plants. We have developed a modification of the single-cell sampling technique to investigate GLR co-expression, and thus potential heteromer formation, in single cells of Arabidopsis thaliana leaves. Micro-EXpression amplification (MEX) has allowed us to amplify gene transcripts from a single cell, enabling expression of up to 100 gene transcripts to be assayed. We measured, on average, the transcripts of five to six different AtGLRs in a single cell. However, no consistent patterns of co-expression or cell-type-specific expression were detected, except that cells sampled from the same plant showed similar expression profiles. The only discernible feature was the detection of AtGLR3.7 in every cell examined, an observation supported by GUS staining patterns in plants stably expressing promoter::uidA fusions. In addition, we found AtGLR3.7 expression in oocytes induces a Ba2+-, Ca2+- and Na+-permeable plasma membrane conductance.
Keywords: AtGLR3.7
glutamate receptor-like genes
micro-EXpression amplification
single-cell sampling
twoelectrode voltage clamp
Xenopus oocytes.
DOI: 10.1111/j.1365-3040.2008.01801.x
Published version:
Appears in Collections:Agriculture, Food and Wine publications
Aurora harvest 5

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.