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|Title:||Recombinant fowlpox virus elicits transient cytotoxic T cell responses due to suboptimal innate recognition and recruitment of T cell help|
|Citation:||Vaccine, 2008; 26(29-30):3566-3573|
|Publisher:||Elsevier Sci Ltd|
|Kerrilyn R. Diener, Erin L. Lousberg, Emma L. Beukema, Anastasia Yu, Paul M. Howley, Michael P. Brown and John D. Hayball|
|Abstract:||Recombinant fowlpox viruses (FPVs) have been used in a variety of vaccine strategies; however strong data clearly demonstrating the characteristics of the strength and nature of the resultant immune response elicited by these vectors are lacking. By utilising a recombinant variant of FPV which expresses the nominal antigen chicken ovalbumin (OVA), and assessing innate FPV- and OVA-specific adaptive immune responses, we show that recombinant FPV induces a rapid type I interferon (IFN) response, mediated primarily by plasmacytoid dendritic cells (pDCs). These cells are necessary for the development of a strong but transient CD8(+) T cell effector response directed against OVA-expressing target cells. We propose that a combination of suboptimal type I IFN production, poor CD4(+) T cell helper function and inefficient DC licensing likely contribute to this transient response. These findings now provide a sound basis for rational modifications to be made to recombinant FPV, designed to improve subsequent vaccine responses.|
|Keywords:||Dendritic Cells; CD4-Positive T-Lymphocytes; T-Lymphocytes, Helper-Inducer; T-Lymphocytes, Cytotoxic; Animals; Mice, Inbred C57BL; Mice; Fowlpox virus; Ovalbumin; Vaccines, Synthetic; Viral Vaccines; Interferon-gamma|
|Description:||Copyright © 2008 Elsevier Ltd All rights reserved.|
|Appears in Collections:||Medicine publications|
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