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https://hdl.handle.net/2440/53424
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Type: | Journal article |
Title: | A small component of the endoplasmic reticulum is required for store-operated Ca2+ channel activation in liver cells: evidence from studies using TRPV1 and taurodeoxycholic acid |
Author: | Castro, J. Aromataris, E. Rychkov, G. Barritt, G. |
Citation: | Biochemical Journal, 2009; 418(Part 3):553-566 |
Publisher: | Portland Press |
Issue Date: | 2009 |
ISSN: | 0264-6021 1470-8728 |
Statement of Responsibility: | Joel Castro, Edoardo C. Aromataris, Grigori Y. Rychkov and Greg J. Barritt |
Abstract: | The question of whether the activation of SOCs (store-operated Ca(2+) channels) requires the whole or part of the ER (endoplasmic reticulum) has not been fully resolved. The role of a putative sub-compartment of the ER in SOC activation in liver cells was investigated using ectopically expressed TRPV1 (transient receptor potential vanilloid 1), a non-selective cation channel, and TDCA (taurodeoxycholic acid), an activator of SOCs, to release Ca(2+) from different regions of the ER. TRPV1 was expressed in the ER and in the plasma membrane. The amount of Ca(2+) released from the ER by a TRPV1 agonist, measured using fura-2, was the same as that released by a SERCA (sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase) inhibitor, indicating that TRPV1 agonist-sensitive stores substantially overlap with SERCA inhibitor-sensitive stores. In contrast with SERCA inhibitors, TRPV1 agonists did not activate store-operated Ca(2+) entry. These findings were confirmed by patch-clamp recording. Using FFP-18, it was shown that SERCA inhibitors release Ca(2+) from the ER located closer to the plasma membrane than the region from which TRPV1 agonists release Ca(2+). In contrast with SERCA inhibitors, TRPV1 agonists did not induce a redistribution of STIM1 (stromal interaction molecule 1). TDCA caused the release of Ca(2+) from the ER, which was detected by FFP-18 but not by fura-2, and a redistribution of STIM1 to puncta similar to that caused by SERCA inhibitors. It is concluded that in liver cells, Ca(2+) release from a small component of the ER located near the plasma membrane is required to induce STIM1 redistribution and SOC activation. |
Keywords: | bile acids calcium release endoplasmic reticulum stromal interaction molecule 1 (STIM1) store-operated calcium entry transient receptor potential vanilloid 1 (TRPV1) |
Description: | Copyright © The Authors Journal compilation © 2009 Biochemical Society |
DOI: | 10.1042/BJ20081052 |
Grant ID: | http://purl.org/au-research/grants/arc/DP0559562 http://purl.org/au-research/grants/arc/DP0559562 |
Published version: | http://dx.doi.org/10.1042/bj20081052 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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