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Type: Journal article
Title: A small component of the endoplasmic reticulum is required for store-operated Ca2+ channel activation in liver cells: evidence from studies using TRPV1 and taurodeoxycholic acid
Author: Castro, J.
Aromataris, E.
Rychkov, G.
Barritt, G.
Citation: Biochemical Journal, 2009; 418(Part 3):553-566
Publisher: Portland Press
Issue Date: 2009
ISSN: 0264-6021
Statement of
Joel Castro, Edoardo C. Aromataris, Grigori Y. Rychkov and Greg J. Barritt
Abstract: The question of whether the activation of SOCs (store-operated Ca(2+) channels) requires the whole or part of the ER (endoplasmic reticulum) has not been fully resolved. The role of a putative sub-compartment of the ER in SOC activation in liver cells was investigated using ectopically expressed TRPV1 (transient receptor potential vanilloid 1), a non-selective cation channel, and TDCA (taurodeoxycholic acid), an activator of SOCs, to release Ca(2+) from different regions of the ER. TRPV1 was expressed in the ER and in the plasma membrane. The amount of Ca(2+) released from the ER by a TRPV1 agonist, measured using fura-2, was the same as that released by a SERCA (sarcoplasmic/endoplasmic reticulum Ca(2+)-ATPase) inhibitor, indicating that TRPV1 agonist-sensitive stores substantially overlap with SERCA inhibitor-sensitive stores. In contrast with SERCA inhibitors, TRPV1 agonists did not activate store-operated Ca(2+) entry. These findings were confirmed by patch-clamp recording. Using FFP-18, it was shown that SERCA inhibitors release Ca(2+) from the ER located closer to the plasma membrane than the region from which TRPV1 agonists release Ca(2+). In contrast with SERCA inhibitors, TRPV1 agonists did not induce a redistribution of STIM1 (stromal interaction molecule 1). TDCA caused the release of Ca(2+) from the ER, which was detected by FFP-18 but not by fura-2, and a redistribution of STIM1 to puncta similar to that caused by SERCA inhibitors. It is concluded that in liver cells, Ca(2+) release from a small component of the ER located near the plasma membrane is required to induce STIM1 redistribution and SOC activation.
Keywords: bile acids
calcium release
endoplasmic reticulum
stromal interaction molecule 1 (STIM1)
store-operated calcium entry
transient receptor potential vanilloid 1 (TRPV1)
Description: Copyright © The Authors Journal compilation © 2009 Biochemical Society
DOI: 10.1042/BJ20081052
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Appears in Collections:Aurora harvest 5
Molecular and Biomedical Science publications

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