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https://hdl.handle.net/2440/53501
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dc.contributor.author | Munro, K. | - |
dc.contributor.author | Mariana, A. | - |
dc.contributor.author | Klavins, A. | - |
dc.contributor.author | Foster, A. | - |
dc.contributor.author | Lai, B. | - |
dc.contributor.author | Vogt, S. | - |
dc.contributor.author | Cai, Z. | - |
dc.contributor.author | Harris, H. | - |
dc.contributor.author | Dillon, C. | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Chemical Research in Toxicology, 2008; 21(9):1760-1769 | - |
dc.identifier.issn | 0893-228X | - |
dc.identifier.issn | 1520-5010 | - |
dc.identifier.uri | http://hdl.handle.net/2440/53501 | - |
dc.description | Copyright © 2008 American Chemical Society | - |
dc.description.abstract | Arsenic (As) is responsible for mass-poisonings worldwide following the ingestion of As-contaminated drinking water. Importantly, however, As toxicity is exploited in the antileukemia drug, Trisenox (As2O3), which successfully cures 65-80% of patients suffering relapsed acute promyelocytic leukemia. In an effort to determine the intracellular organelle and biomolecular targets of As, microprobe X-ray fluorescence (XRF) and X-ray absorption spectroscopy (XAS) analyses were performed on HepG2 cells following their exposure to high doses of arsenite (1 mM) or arsenate (20 mM). Microprobe XRF elemental mapping of thin-sectioned cells showed As accumulation in the euchromatin region of the cell nucleus (following arsenite exposure) synonymous with As targeting of DNA or proteins involved in DNA transcription. X-ray absorption near edge spectroscopy (XANES) and extended X-ray absorption fine structure (EXAFS) analysis of arsenite-treated cells, however, showed the predominance of an As tris-sulfur species, providing increased credence to As interactions with nuclear proteins as a key factor in As-induced toxicity. | - |
dc.description.statementofresponsibility | Kristie L. Munro, Anna Mariana, Andrejs I. Klavins, Amalanie J. Foster, Barry Lai, Stefan Vogt, ZhongHou Cai, Hugh H. Harris and Carolyn T. Dillon | - |
dc.language.iso | en | - |
dc.publisher | Amer Chemical Soc | - |
dc.source.uri | http://dx.doi.org/10.1021/tx800128d | - |
dc.subject | Cell Line, Tumor | - |
dc.subject | Cell Nucleus | - |
dc.subject | Humans | - |
dc.subject | Arsenates | - |
dc.subject | Arsenites | - |
dc.subject | Electron Probe Microanalysis | - |
dc.subject | Spectrometry, X-Ray Emission | - |
dc.subject | Drug Screening Assays, Antitumor | - |
dc.subject | Inhibitory Concentration 50 | - |
dc.subject | Cell Survival | - |
dc.subject | Molecular Structure | - |
dc.subject | Dose-Response Relationship, Drug | - |
dc.subject | Time Factors | - |
dc.title | Microprobe XRF mapping and XAS investigations of the intracellular metabolism of arsenic for understanding arsenic-induced toxicity | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1021/tx800128d | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Harris, H. [0000-0002-3472-8628] | - |
Appears in Collections: | Aurora harvest 5 Chemistry and Physics publications Environment Institute publications |
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