Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/53821
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Type: Journal article
Title: Faecal microflora and β-glucuronidase expression are altered in an irinotecan-induced diarrhea model in rats
Other Titles: Faecal microflora and beta-glucuronidase expression are altered in an irinotecan-induced diarrhea model in rats
Author: Stringer, A.
Gibson, R.
Logan, R.
Bowen, J.
Yeoh, A.
Keefe, D.
Citation: Cancer Biology & Therapy, 2008; 7(12):1919-1925
Publisher: Landes Bioscience
Issue Date: 2008
ISSN: 1538-4047
1555-8576
Statement of
Responsibility: 
Andrea M Stringer, Rachel J Gibson, Richard M Logan, Joanne M Bowen, Ann SJ Yeoh and Dorothy MK Keefe
Abstract: OBJECTIVES: Chemotherapy-induced diarrhea (CID) is a well recognized side effect of cancer treatment. However, the pathophysiology behind this debilitating side effect remains unclear. Irinotecan causes cholinergic and delayed onset diarrhea in patients, in which beta-glucuronidase produced by gut bacteria is thought to be involved. RESULTS: Diarrhea occurred, as expected, following irinotecan treatment. beta-glucuronidase expression increased in the jejunum and colon. Faecal flora changed quantitatively after treatment also, with increases in E. coli, Staphylococcus spp. and Clostridium spp. (all beta-glucuronidase producing) and decreases in Lactobacillus spp., Bifidobacterium spp. (both beneficial bacteria), and Bacteroides spp. (beta-glucuronidase producing, major component of intestinal flora). METHODS: Rats were treated with 200 mg/kg irinotecan and killed at various time points up to 72 h. Rats were monitored for diarrhea. Sections were stained for beta-glucuronidase expression, and faecal DNA was analysed using real time PCR. CONCLUSIONS: Irinotecan-induced diarrhea may be caused by an increase in beta-glucuronidase producing bacteria. However, the increase in bacteria may also be caused by irinotecan, further exaggerating the toxicity of the drug and emphasising the need for these specific bacteria to be therapeutically targeted for successful treatment regimens to be accomplished.
Keywords: Gastrointestinal Tract; Intestines; Jejunum; Feces; Animals; Rats; Bacteria; Escherichia coli; Staphylococcus; Lactobacillus; Diarrhea; Camptothecin; Glucuronidase; Antineoplastic Agents, Phytogenic; Immunohistochemistry; Gene Expression Regulation, Enzymologic; Irinotecan
Description: © 2008 Landes Bioscience
RMID: 0020084269
DOI: 10.4161/cbt.7.12.6940
Description (link): http://www.ncbi.nlm.nih.gov/pubmed/18927500
Appears in Collections:Dentistry publications

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